Evidence map›Paper›PMID 39105916›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Exosomes Isolation, Purification, and Characterization.

Prabhat Ranjan, Suresh Kumar Verma

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Exosomes in ocular graft-versus-host disease: an overview.International journal of ophthalmology · 2026
    Review
  3. Review
  4. Advances in the Role of Stem Cell-Derived Exosomes in Diabetic Foot Wound Healing.Diabetes, metabolic syndrome and obesity : targets and therapy · 2025
    Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Prabhat RanjanDepartment of Medicine, Division of Cardiovascular Disease, The University of Alabama at Birmingham, Birmingham, AL, USA.
Suresh Kumar VermaDepartment of Medicine, Division of Cardiovascular Disease, The University of Alabama at Birmingham, Birmingham, AL, USA. sverma@uabmc.edu.

Funding

IL10 REGULATION OF FIBROBLAST PROGENITOR CELL MEDIATED CARDIAC FIBROSISR01HL135060 · NHLBI · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI VERMA, SURESH K · 2017 to 2021
$1.9M
American Heart Association-American Stroke Association 958292American Heart Association-Career Development Award 23CDA1047979NHLBI NIH HHS R01 HL135060
6 · The paper itself

Abstract

Exosomes are double-layered lipid membranous nanovesicles that are endosomal in origin and secreted by almost all cells. They are 30-130 nm in size and contain various molecular signatures such as miRNAs, mRNAs, DNA, lipids, and proteins. Due to their highly heterogeneous content, exosomes have a major role in influencing cellular physiology and pathology. Although exosome research has been in progress for a long time, its biomedical applications have recently been expanding due to its bio-friendly nature. However, the most challenging part is its isolation to obtain quality exosomes with good yield. Therefore, in this chapter, we have described appropriate protocols for exosome isolation and characterization along with alternative purification methods.

Indexed as

ExosomesCell FractionationHumansUltracentrifugationBone marrow-progenitor cellsEndothelial cellsExosomesFibroblastsSucrose density gradientUltracentrifugation

Identifiers

PMID39105916

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.