Evidence map›Paper›PMID 39104301›Full record

ArticleLab on a chip2024

Micro blood analysis technology (μBAT): multiplexed analysis of neutrophil phenotype and function from microliter whole blood samples.

Terry D Juang, Jeremiah Riendeau, Peter G Geiger, Rupsa Datta, Marcos Lares, Ravi Chandra Yada, Anne Marie Singh, Christine M Seroogy, James E Gern, Melissa C Skala and 2 more

Abstract read
In one paragraph

Article in Lab on a chip, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Terry D JuangDepartment of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA. djbeebe@wisc.edu.ORCID 0000-0002-0163-537X
Jeremiah RiendeauMorgridge Institute for Research, Madison, WI, USA. skerr2@wisc.edu.ORCID 0000-0002-3014-2849
Peter G GeigerDepartment of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, USA.
Rupsa DattaMorgridge Institute for Research, Madison, WI, USA. skerr2@wisc.edu.ORCID 0000-0002-6432-2389
Marcos LaresDepartment of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, USA.
Ravi Chandra YadaDepartment of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, USA.ORCID 0000-0002-5385-1728
Anne Marie SinghDepartment of Pediatrics, School of Medicine and Public Health, University of Wisconsin-Madison, WI, USA.
Christine M SeroogyDepartment of Pediatrics, School of Medicine and Public Health, University of Wisconsin-Madison, WI, USA.
James E GernDepartment of Pediatrics, School of Medicine and Public Health, University of Wisconsin-Madison, WI, USA.
Melissa C SkalaDepartment of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA. djbeebe@wisc.edu.ORCID 0000-0002-6320-7637
David J BeebeDepartment of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA. djbeebe@wisc.edu.
Sheena C KerrDepartment of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA. djbeebe@wisc.edu.ORCID 0000-0002-4404-3382

Funding

UW COMPREHENSIVE CANCER CENTER SUPPORTP30CA014520 · NCI · UNIVERSITY OF WISCONSIN-MADISON · PI Justine Yang Bruce · 1985 to 2026
$142.6M
Functional optical imaging for rapid, label-free predictions of treatment response and clonal evolution in patient-derived cancer organoidsR01CA272855 · NCI · MORGRIDGE INSTITUTE FOR RESEARCH, INC. · PI Dustin A Deming, Melissa Caroline Skala · 2023 to 2026
$3.1M
Label-free imaging of CAR T cell metabolismR01CA278051 · NCI · MORGRIDGE INSTITUTE FOR RESEARCH, INC. · PI Christian Capitini, Krishanu Saha · 2023 to 2026
$2.6M
Label-free single-cell imaging for quality control of cardiomyocyte biomanufacturingR01HL165726 · NHLBI · MORGRIDGE INSTITUTE FOR RESEARCH, INC. · PI Sean P Palecek, Melissa Caroline Skala · 2023 to 2026
$2.5M
A multiplexed micro scale assay for real time analysis of pediatric immune cell functionU24AI152177 · NIAID · UNIVERSITY OF WISCONSIN-MADISON · PI BEEBE, DAVID J, KERR, SHEENA CATHERINE · 2020 to 2024
$1.8M
NCI NIH HHS P30 CA014520NCI NIH HHS R01 CA272855NCI NIH HHS R01 CA278051NHLBI NIH HHS R01 HL165726NIAID NIH HHS U24 AI152177
6 · The paper itself

Abstract

There is an ongoing need to do more with less and provide highly multiplexed analysis from limited sample volumes. Improved "sample sparing" assays would have a broad impact across pediatric and other rare sample type studies in addition to enabling sequential sampling. This capability would advance both clinical and basic research applications. Here we report the micro blood analysis technology (μBAT), a microfluidic platform that supports multiplexed analysis of neutrophils from a single drop of blood. We demonstrate the multiplexed orthogonal capabilities of μBAT including functional assays (phagocytosis, neutrophil extracellular traps, optical metabolic imaging) and molecular assays (gene expression, cytokine secretion). Importantly we validate our microscale platform using a macroscale benchmark assay. μBAT is compatible with lancet puncture or microdraw devices, and its design facilitates rapid operations without the need for specialized equipment. μBAT offers a new method for investigating neutrophil function in populations with restricted sample amounts.

Indexed as

NeutrophilsCytokinesEquipment DesignHumansLab-On-A-Chip DevicesMicrofluidic Analytical TechniquesPhenotypeCytokines

Identifiers

PMID39104301
PMCPMC11335436

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.