ArticleNature communications2024
Multiplexed volumetric CLEM enabled by scFvs provides insights into the cytology of cerebellar cortex.
Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Probing molecular diversity and ultrastructure of brain cells with fluorescent aptamers.Nature communications · 2026Article
- Deciphering Cellular Intricacies for Drug Discovery: A Synergistic Approach Combining Cryo-CLEM, Electromechanical Modeling, a nd AI-Guided Simulations.Combinatorial chemistry & high throughput screening · 2026Article
- Structured sampling of molecularly classified mossy fiber inputs by cerebellar granule cells.Frontiers in computational neuroscience · 2026Article
- MitoEM 2.0: A Benchmark for Challenging 3D Mitochondria Instance Segmentation from EM Images.bioRxiv : the preprint server for biology · 2025Article
- Open-source antibodies as a path to enhanced research reproducibility and transparency.New biotechnology · 2025Article
- Article
- Genetically encoded intrabody probes for labeling and manipulating AMPA-type glutamate receptors.Nature communications · 2024Article
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21 authors.
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Abstract
Mapping neuronal networks is a central focus in neuroscience. While volume electron microscopy (vEM) can reveal the fine structure of neuronal networks (connectomics), it does not provide molecular information to identify cell types or functions. We developed an approach that uses fluorescent single-chain variable fragments (scFvs) to perform multiplexed detergent-free immunolabeling and volumetric-correlated-light-and-electron-microscopy on the same sample. We generated eight fluorescent scFvs targeting brain markers. Six fluorescent probes were imaged in the cerebellum of a female mouse, using confocal microscopy with spectral unmixing, followed by vEM of the same sample. The results provide excellent ultrastructure superimposed with multiple fluorescence channels. Using this approach, we documented a poorly described cell type, two types of mossy fiber terminals, and the subcellular localization of one type of ion channel. Because scFvs can be derived from existing monoclonal antibodies, hundreds of such probes can be generated to enable molecular overlays for connectomic studies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.