Evidence map›Paper›PMID 39102354›Full record

ArticleACS applied bio materials2024

Interrogating the Role of Endocytosis Pathway and Organelle Trafficking for Doxorubicin-Based Combination Ionic Nanomedicines.

Mujeebat Bashiru, Muhammad Rayaan, Nawab Ali, Samir V Jenkins, Adeniyi Oyebade, Md Shahedur Rahman, Robert J Griffin, Adegboyega K Oyelere, Noureen Siraj

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS applied bio materials, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Mujeebat BashiruDepartment of Chemistry, University of Arkansas at Little Rock, Little Rock, Arkansas 72204, United States.ORCID 0000-0002-7947-5361
Muhammad RayaanDepartment of Chemistry, University of Arkansas at Little Rock, Little Rock, Arkansas 72204, United States.
Nawab AliDepartment of Biology, University of Arkansas at Little Rock, Little Rock, Arkansas 72204, United States.
Samir V JenkinsDepartment of Radiation Oncology, Winthrop P. Rockefeller Cancer Institute, University of Arkansas for Medical Sciences, 4301 W Markham St, Little Rock, Arkansas 72205, United States.ORCID 0000-0003-1496-2035
Adeniyi OyebadeDepartment of Chemistry, University of Arkansas at Little Rock, Little Rock, Arkansas 72204, United States.
Md Shahedur RahmanDepartment of Chemistry, University of Arkansas at Little Rock, Little Rock, Arkansas 72204, United States.
Robert J GriffinDepartment of Radiation Oncology, Winthrop P. Rockefeller Cancer Institute, University of Arkansas for Medical Sciences, 4301 W Markham St, Little Rock, Arkansas 72205, United States.
Adegboyega K OyelereSchool of Chemistry and Biochemistry, Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia 30332, United States.
Noureen SirajDepartment of Chemistry, University of Arkansas at Little Rock, Little Rock, Arkansas 72204, United States.ORCID 0000-0003-4925-0265

Funding

Understanding Hesitant AdoptersP20GM103429 · NIGMS · UNIV OF ARKANSAS FOR MED SCIS · PI Lawrence E Cornett · 2012 to 2026
$60.9M
NIGMS NIH HHS P20 GM103429
6 · The paper itself

Abstract

We have studied the endocytic mechanisms that determine subcellular localization for three carrier-free chemotherapeutic-photothermal (chemo-PTT) combination ionic nanomedicines (INMs) composed of doxorubicin (DOX) and an near-infrared (NIR) dye (ICG, IR820, or IR783). This study aims to understand the cellular basis for previously published enhanced toxicity results of these combination nanomedicines toward MCF-7 breast cancer cells. The active transport mechanism of INMs, unlike free DOX, which is known to employ passive transport, was validated by conducting temperature-dependent cellular uptake of the drug in MCF-7 cells using confocal microscopy. The internalization pathway of these INMs was further probed in the presence and absence of different endocytosis inhibitors. Detailed examination of the mode of entry of the carrier-free INMs in MCF-7 cells revealed that they are primarily internalized through clathrin-mediated endocytosis. In addition, time-dependent subcellular localization studies were also investigated. Examination of time-dependent confocal images indicated that the INMs targeted multiple organelles, in contrast to free DOX that primarily targets the nucleus. Collectively, the high cellular endocytic uptake in cancerous cells (EPR effect) and the multimode targeting ability demonstrated the main reason for the low half-maxima inhibitory concentration (IC

Indexed as

DoxorubicinEndocytosisNanomedicineAntibiotics, AntineoplasticBiocompatible MaterialsCell SurvivalDrug Screening Assays, AntitumorHumansIonsMaterials TestingMCF-7 CellsOrganellesParticle SizeAntibiotics, AntineoplasticBiocompatible MaterialsDoxorubicinIonscellular uptakedoxorubicinendocytosis inhibitorsionic nanomaterialsubcellular localization

Identifiers

PMID39102354
PMCPMC11457535

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.