Evidence map›Paper›PMID 39101070›Full record

ArticleJournal of advanced veterinary and animal research2024

Characterization of the dominant strain (G-VII) of Newcastle disease viruses isolated from commercial chickens in Bangladesh during recent outbreaks.

Mohammad Aynul Haque, Mohammad Sadekuzzaman, Md Enamul Haque, Mst Kohinoor Parvin, Md Mostofa Kamal, Sajedul Hayat, Md Ariful Islam, Mst Minara Khatun, Mahbubul Pratik Siddique, Sham Soun Nahar and 3 more

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In one paragraph

Article in Journal of advanced veterinary and animal research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

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0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Mohammad Aynul HaqueDepartment of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.ORCID https://orcid.org/0000-0002-9293-9642
Mohammad SadekuzzamanCentral Disease Investigation Laboratory, Department of Livestock Services, Dhaka, Bangladesh.ORCID https://orcid.org/0000-0002-9293-9642
Md Enamul HaqueDepartment of Microbiology, Sheikh Hasina University of Science and Technology, Bhairab, Bangladesh.ORCID https://orcid.org/0000-0002-9293-9642
Mst Kohinoor ParvinDepartment of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.ORCID https://orcid.org/0000-0002-9293-9642
Md Mostofa KamalDepartment of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.ORCID https://orcid.org/0000-0002-9293-9642
Sajedul HayatDepartment of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.ORCID https://orcid.org/0000-0002-9293-9642
Md Ariful IslamDepartment of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.ORCID https://orcid.org/0000-0002-9293-9642
Mst Minara KhatunDepartment of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.ORCID https://orcid.org/0000-0002-9293-9642
Mahbubul Pratik SiddiqueDepartment of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.ORCID https://orcid.org/0000-0002-9293-9642
Sham Soun NaharLivestock Research Institute, Mohakhali, Dhaka, Bangladesh.ORCID https://orcid.org/0000-0002-9293-9642
A K M KhasruzzamanDepartment of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.ORCID https://orcid.org/0000-0002-9293-9642
Muhammud Tofazzal HossainDepartment of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.ORCID https://orcid.org/0000-0002-9293-9642
Md Alimul IslamDepartment of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.ORCID https://orcid.org/0000-0002-9293-9642

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Objective: Newcastle disease virus genotype VII (NDV-GVII), an extremely infectious pathogen, has been causing severe economic consequences for the chicken industry. The current study aimed to isolate and characterize NDV-GVII from commercial chickens in Bangladesh during a recent outbreak. Materials and Methods: From clinically suspected chickens from 70 commercial poultry farms, a total of 420 samples (trachea, lungs, and brain tissue) were collected. The samples were cultivated in 9-10 day-old seronegative embryonated chicken eggs (ECEs) after evaluating them using the rapid Newcastle disease virus (NDV) antigen detection kit. The hemagglutination (HA) inhibition test, agar gel immune diffusion (AGID) test, molecular detection by reverse transcription-polymerase chain reaction (RT-PCR), and phylogenetic studies using gene sequences of fusion (F) protein. The HA pattern of isolated NDV was determined using different avian and mammalian red blood cells (RBCs). The pathogenicity of the isolated virus was evaluated using mean death time (MDT), intravenous pathogenicity index (IVPI), and intracerebral pathogenicity index (ICPI). Results: The study found 87 NDV samples positive using the rapid NDV Ag detection kit and then 60 positives for virus isolation in ECEs. All 60 isolates were positive for NDV by HI, AGID, and RT-PCR. Phylogenetic tree analysis indicated that recent NDV isolates belong to genotype VII and exhibit a similarity of 99.7%-98.5% with isolates from Bangladesh, Iran, and India. The new isolates, identified as velogenic strains of NDV, possess an F protein cleavage site with Conclusion: This study concluded that NDV-GVII is prevalent in commercial poultry farms in Bangladesh.

Indexed as

ICPIIVPIMDT genotype VIINDVRT-PCR

Identifiers

PMID39101070
PMCPMC11296175

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