Evidence map›Paper›PMID 39100595›Full record

ArticleBio-protocol2024

Purification and Cryo-Electron Microscopy Analysis of Bacterial Appendages.

Juan C Sanchez, Joseph K Baumgardt, Elizabeth R Wright

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Juan C SanchezDepartment of Biochemistry, University of Wisconsin-Madison, Madison, WI, USA.
Joseph K BaumgardtDepartment of Biochemistry, University of Wisconsin-Madison, Madison, WI, USA.
Elizabeth R WrightDepartment of Biochemistry, University of Wisconsin-Madison, Madison, WI, USA.

Funding

The Midwest Center for Cryo-Electron TomographyU24GM139168 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI WRIGHT, ELIZABETH R · 2020 to 2025
$41.7M
Biotechnology Training ProgramT32GM135066 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI SCOTT M. COYLE, BRIAN G FOX · 2020 to 2026
$7.0M
Structure and Function of Prokaryotic Appendages by Cryo-Electron TomographyR01GM104540 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI WRIGHT, ELIZABETH R · 2013 to 2017
$1.4M
NIGMS NIH HHS R01 GM104540NIGMS NIH HHS T32 GM135066NIGMS NIH HHS U24 GM139168
6 · The paper itself

Abstract

A number of extracellular helical protein polymers are crucial for supporting bacterial motility. The bacterial flagellum is a polymeric appendage used to support cellular motility. Historically, structural studies of flagellar and other filaments were limited to those present as or locked into straightened states. Here, we present a robust workflow that produces biologically relevant high-resolution cryo-electron microscopy (cryo-EM) structures of bacterial flagellar filaments. We highlight how a simple purification method, centered around several centrifugation steps, exploits the process of filament ejection in

Indexed as

Bacterial flagellumCryo-EMHelical reconstructionPolymersPurificationVitrification

Identifiers

PMID39100595
PMCPMC11292135

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.