Evidence map›Paper›PMID 39096359›Full record

ArticleParasitology research2024

Molecular and serological prevalence rates of Neospora caninum infection in dogs from Jordan.

Rami M Mukbel, Areen A Okour, Marwan M Abu-Halaweh, Haifa B Hammad, Mahmoud N Abo-Shehada

Abstract read
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In one paragraph

Article in Parasitology research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Seroprevalence ofAnimals : an open access journal from MDPI · 2026
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Rami M MukbelDepartment of Basic Veterinary Medical Science, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, Jordan. rmmukbel@just.edu.jo.ORCID http://orcid.org/0000-0002-1985-5717
Areen A OkourDepartment of Basic Veterinary Medical Science, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, Jordan.
Marwan M Abu-HalawehDepartment of Biotechnology and Genetic Engineering, Philadelphia University, Amman, Jordan.
Haifa B HammadDepartment of Basic Veterinary Medical Science, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, Jordan.
Mahmoud N Abo-ShehadaFaculty of Epidemiology and Population Health, London School of Hygiene and Tropical Medicine, University of London, London, UK. Mahmoud.abo-shehada@lshtm.ac.uk.ORCID http://orcid.org/0000-0001-8045-5256

Funding

Deanship of Research, Jordan University of Science and Technology 215/2021
6 · The paper itself

Abstract

Neosporosis is a proven disease of farm animals and dogs caused by Neospora caninum. This cross-sectional study investigates N. caninum prevalence and seroprevalence among 268 dogs. Nc5 gene PCR was carried out on dog faeces and confirmed by sequencing. Seroprevalence was detected using an indirect fluorescent antibody test (IFAT). Three age groups, gender, locality (Amman, Irbid, and Zarqa Governorates), dog type (stray, pet, and breeding), place of living (indoor/outdoor), food type (raw/cooked), having diarrhoea, having abortion in the area, and having animals nearby were tested as independent variables for associations with positivity to N. caninum using univariate and multivariable logistic regression analyses. The true prevalence of N. caninum was 34.3% (95% CI 28.4, 40.5) using the Nc5-PCR test. The true seroprevalence rate of N. caninum among dogs in Jordan was 47.9% (95% CI 41.4, 54.5) using IFAT. The sequenced isolates of Nc5-PCR products (n = 85) matched three N. caninum strains, namely, NcHareGre (n = 70, 82.4%, 95% CI 72.6-89), NC MS2 (n = 14, 16.5%, 95% CI 9.3-26.1), and L218 (n = 1, 1.2%, 95% CI 0.03-6.4). The three strains were isolated previously from three different countries and continents. N. caninum shedding is associated with abortion among dogs and animals in the area (odds ratio = 3.6). In Amman and Zarqa, living indoors reduced seroprevalence at 0.45, 0.24, and 0.02 odds ratios, respectively. Jordan shares three molecular N. caninum strains with three different countries and continents.

Indexed as

CoccidiosisDog DiseasesFecesNeosporaAnimalsAntibodies, ProtozoanCross-Sectional StudiesDogsFemaleFluorescent Antibody Technique, IndirectJordanMalePolymerase Chain ReactionPrevalenceSeroepidemiologic StudiesAntibodies, ProtozoanDogsIFATJordanNeospora caninumPCRPrevalence

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.