Evidence map›Paper›PMID 39095803›Full record

ArticleBMC oral health2024

Fibrin-konjac glucomannan-black phosphorus hydrogel scaffolds loaded with nasal ectodermal mesenchymal stem cells accelerated alveolar bone regeneration.

Yin Zou, Xue Mei, Xinhe Wang, Xuan Zhang, Xun Wang, Wen Xiang, Naiyan Lu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC oral health, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yin ZouDepartment of Stomatology, Affiliated Children's Hospital of Jiangnan University, Jiangnan University, Wuxi, Jiangsu Province, People's Republic of China.
Xue MeiSchool of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu Province, People's Republic of China.
Xinhe WangSchool of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu Province, People's Republic of China.
Xuan ZhangSchool of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu Province, People's Republic of China.
Xun WangJiangnan University Medical Center, Wuxi, Jiangsu Province, People's Republic of China.
Wen XiangDepartment of Hepatobiliary Surgery, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu Province, People's Republic of China.
Naiyan LuSchool of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu Province, People's Republic of China. lunaiyan@jiangnan.edu.cn.

Funding

Wuxi Health Committee No. HB2023093Wuxi Health Committee No. Q202102
6 · The paper itself

Abstract

backgroundEffective treatments for the alveolar bone defect remain a major concern in dental therapy. The objectives of this study were to develop a fibrin and konjac glucomannan (KGM) composite hydrogel as scaffolds for the osteogenesis of nasal mucosa-derived ectodermal mesenchymal stem cells (EMSCs) for the regeneration of alveolar bone defect, and to investigate the osteogenesis-accelerating effects of black phosphorus nanoparticles (BPNs) embedded in the hydrogels.

methodsPrimary EMSCs were isolated from rat nasal mucosa and used for the alveolar bone recovery. Fibrin and KGM were prepared in different ratios for osteomimetic hydrogel scaffolds, and the optimal ratio was determined by mechanical properties and biocompatibility analysis. Then, the optimal hydrogels were integrated with BPNs to obtain BPNs/fibrin-KGM hydrogels, and the effects on osteogenic EMSCs in vitro were evaluated. To explore the osteogenesis-enhancing effects of hydrogels in vivo, the BPNs/fibrin-KGM scaffolds combined with EMSCs were implanted to a rat model of alveolar bone defect. Micro-computed tomography (CT), histological examination, real-time quantitative polymerase chain reaction (RT-qPCR) and western blot were conducted to evaluate the bone morphology and expression of osteogenesis-related genes of the bone regeneration.

resultsThe addition of KGM improved the mechanical properties and biodegradation characteristics of the fibrin hydrogels. In vitro, the BPNs-containing compound hydrogel was proved to be biocompatible and capable of enhancing the osteogenesis of EMSCs by upregulating the mineralization and the activity of alkaline phosphatase. In vivo, the micro-CT analysis and histological evaluation demonstrated that rats implanted EMSCs-BPNs/fibrin-KGM hydrogels exhibited the best bone reconstruction. And compared to the model group, the expression of osteogenesis genes including osteopontin (Opn, p < 0.0001), osteocalcin (Ocn, p < 0.0001), type collagen (Col , p < 0.0001), bone morphogenetic protein-2 (Bmp2, p < 0.0001), Smad1 (p = 0.0006), and runt-related transcription factor 2 (Runx2, p < 0.0001) were all significantly upregulated.

conclusionsEMSCs/BPNs-containing fibrin-KGM hydrogels accelerated the recovery of the alveolar bone defect in rats by effectively up-regulating the expression of osteogenesis-related genes, promoting the formation and mineralisation of bone matrix.

Indexed as

Bone RegenerationFibrinHydrogelsMannansMesenchymal Stem CellsOsteogenesisPhosphorusRats, Sprague-DawleyTissue ScaffoldsAlveolar ProcessAnimalsBone Morphogenetic Protein 2Core Binding Factor Alpha 1 SubunitMaleNanoparticlesNasal Mucosa(1-6)-alpha-glucomannanBone Morphogenetic Protein 2Core Binding Factor Alpha 1 SubunitFibrinHydrogelsMannansOsteocalcinPhosphorusRunx2 protein, ratAlveolar bone defectBlack phosphorusEctodermal mesenchymal stem cellsFibrinKonjac glucomannan

Identifiers

PMID39095803
PMCPMC11297757

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.