ArticleBMC plant biology2024
Transcriptomic analyses provide molecular insight into the cold stress response of cold-tolerant alfalfa.
Article in BMC plant biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- The bHLH Transcription FactorPlants (Basel, Switzerland) · 2026Article
- Overexpression of an Alfalfa Gene,Genes · 2026Article
- Gene Expression Profiling of Adipose Tissue in Enshi Black Pigs Subjected to Cold Stress.Veterinary sciences · 2026Article
- A Phase-Dependent Model of Sorghum (Biology · 2026Article
- Molecular Mechanisms of Cold Stress Response in Strawberry and Breeding Strategies.Current issues in molecular biology · 2025Review
- Glycosylated sterols enhance cold tolerance in tomato via membrane stabilization and jasmonate signaling.Plant physiology · 2025Article
- Metabolomic Analysis of Plant Hormone-Related Metabolites inMolecules (Basel, Switzerland) · 2025Article
- Advances in basic biology of alfalfa (Horticulture research · 2025Article
- Screening and Identification of Drought-Tolerant Genes in Tomato (Plants (Basel, Switzerland) · 2025Article
- Combined transcriptomic and proteomic analysis reveals the response mechanisms of alfalfa to freezing stress.Frontiers in plant science · 2025Article
- Analysis of physiological characteristics and gene co-expression networks inFrontiers in plant science · 2025Article
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8 authors.
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Abstract
backgroundDaye No.3 is a novel cultivar of alfalfa (Medicago sativa L.) that is well suited for cultivation in high-altitude regions such as the Qinghai‒Tibet Plateau owing to its high yield and notable cold resistance. However, the limited availability of transcriptomic information has hindered our investigation into the potential mechanisms of cold tolerance in this cultivar. Consequently, we conducted de novo transcriptome assembly to overcome this limitation. Subsequently, we compared the patterns of gene expression in Daye No. 3 during cold acclimatization and exposure to cold stress at various time points.
resultsA total of 15 alfalfa samples were included in the transcriptome assembly, resulting in 141.97 Gb of clean bases. A total of 441 DEGs were induced by cold acclimation, while 4525, 5016, and 8056 DEGs were identified at 12 h, 24 h, and 36 h after prolonged cold stress at 4 °C, respectively. The consistency between the RT‒qPCR and transcriptome data confirmed the accuracy and reliability of the transcriptomic data. KEGG enrichment analysis revealed that many genes related to photosynthesis were enriched under cold stress. STEM analysis demonstrated that genes involved in nitrogen metabolism and the TCA cycle were consistently upregulated under cold stress, while genes associated with photosynthesis, particularly antenna protein genes, were downregulated. PPI network analysis revealed that ubiquitination-related ribosomal proteins act as hub genes in response to cold stress. Additionally, the plant hormone signaling pathway was activated under cold stress, suggesting its vital role in the cold stress response of alfalfa.
conclusionsUbiquitination-related ribosomal proteins induced by cold acclimation play a crucial role in early cold signal transduction. As hub genes, these ubiquitination-related ribosomal proteins regulate a multitude of downstream genes in response to cold stress. The upregulation of genes related to nitrogen metabolism and the TCA cycle and the activation of the plant hormone signaling pathway contribute to the enhanced cold tolerance of alfalfa.
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