Evidence map›Paper›PMID 39095443›Full record

ArticleScientific reports2024

Comprehensive characterization of protease inhibiting gene family, cis-regulatory elements, and protein interaction network in linseed and their expression upon bud fly infestation.

Chandra Mohan Singh, Bhupendra Kumar Singh, Shalini Purwar, Beena Nair, Ruchi, Amar Patel, Saurabh Singh, Vikender Kaur

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PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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1 · What the graph read from it

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Chandra Mohan SinghDepartment of Genetics and Plant Breeding, Banda University of Agriculture and Technology, Banda, Uttar Pradesh, 210 001, India.
Bhupendra Kumar SinghDepartment of Entomology, Banda University of Agriculture and Technology, Banda, Uttar Pradesh, 210 001, India. bhupendrakumarsingh@gmail.com.
Shalini PurwarDepartment of Basic and Social Sciences, Banda University of Agriculture and Technology, Banda, Uttar Pradesh, 210 001, India.
Beena NairAICRP on Linseed and Mustard, College of Agriculture, Dr. PDKV-Akola, Nagpur, Maharashtra, 440 001, India.
RuchiDepartment of Genetics and Plant Breeding, Banda University of Agriculture and Technology, Banda, Uttar Pradesh, 210 001, India.
Amar PatelAICRP on Linseed and Sesame, Oilseed Research Station, Banda University of Agriculture and Technology, Mauranipur, Uttar Pradesh, 282 204, India.
Saurabh SinghAICRP on Linseed and Sesame, Oilseed Research Station, Banda University of Agriculture and Technology, Mauranipur, Uttar Pradesh, 282 204, India.
Vikender KaurDivision of Germplasm Evaluation, Indian Council of Agricultural Research-National Bureau of Plant Genetic Resources (ICAR-NBPGR), New Delhi, 110 012, India. kaurvikender@gmail.com.

Funding

Department of Biotechnology, Ministry of Science and Technology, India No. BT/Ag/Network/Linseed/2019-20
6 · The paper itself

Abstract

Linseed, also known as flax is an important oilseed crop with many potential uses in paint, textile, food and pharmaceutical industries. Susceptibility to bud fly (Dasyneura lini Barnes) infestation is a serious biotic concern leading to severe yield penalty in linseed. Protease inhibitors (PIs) are potential candidates that activate during the insect-pest attack and modulate the resistance. In the present study, we explored the PI candidates in the linseed genome and a total of 100 LuPI genes were identified and grouped into five distinct subgroups. The analysis of cis-acting elements revealed that almost all LuPI promoters contain several regulatory elementary related to growth and development, hormonal regulation and stress responses. Across the subfamilies of PIs, the specific domains are consistently found conserved in all protein sequences. The tissue-specific in-silico expression pattern via RNA-seq revealed that all the genes were regulated during different stress. The expression through qRT-PCR of 15 genes revealed the significant up-regulation of LuPI-24, LuPI-40, LuPI-49, LuPI-53, and LuPI-63 upon bud fly infestation in resistant genotype EC0099001 and resistant check variety Neela. This study establishes a foundation resource for comprehending the structural, functional, and evolutionary dimensions of protease inhibitors in linseed.

Indexed as

DipteraFlaxGene Expression Regulation, PlantProtease InhibitorsAnimalsMultigene FamilyPhylogenyPlant DiseasesPlant ProteinsPromoter Regions, GeneticProtein Interaction MapsRegulatory Sequences, Nucleic AcidPlant ProteinsProtease InhibitorsBud flyFlaxGene expressionLinumPhylogenetic analysisProtease inhibitors

Identifiers

PMID39095443
PMCPMC11297176

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.