Evidence map›Paper›PMID 39093424›Full record

ArticleMikrochimica acta2024

Intermittent lysis on a single paper-based device to extract exosomal nucleic acid biomarkers from biological samples for downstream analysis.

Van-Truc Vu, Cao-An Vu, Chun-Jen Huang, Chao-Min Cheng, Shin-Chen Pan, Wen-Yih Chen

Abstract read
PubMed Publisher
In one paragraph

Article in Mikrochimica acta, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Van-Truc VuDepartment of Chemical and Materials Engineering, National Central University, Taoyuan, 320317, Taiwan.
Cao-An VuDepartment of Chemical and Materials Engineering, National Central University, Taoyuan, 320317, Taiwan.
Chun-Jen HuangDepartment of Chemical and Materials Engineering, National Central University, Taoyuan, 320317, Taiwan.
Chao-Min ChengInstitute of Biomedical Engineering, National Tsing Hua University, Hsinchu, 300044, Taiwan.
Shin-Chen PanDepartment of Surgery, Section of Plastic and Reconstructive Surgery, National Cheng Kung University Hospital, National Cheng Kung University, Tainan, 704, Taiwan. pansc@mail.ncku.edu.tw.
Wen-Yih ChenDepartment of Chemical and Materials Engineering, National Central University, Taoyuan, 320317, Taiwan. wychen@ncu.edu.tw.ORCID http://orcid.org/0000-0002-9930-7605

Funding

Ministry of Science and Technology, Taiwan 109-2811-E-008-518Ministry of Science and Technology, Taiwan 111-2314-B-006-093
6 · The paper itself

Abstract

As the role of exosomes in physiological and pathological processes has been properly perceived, harvesting them and their internal components is critical for subsequent applications. This study is a debut of intermittent lysis, which has been integrated into a simple and easy-to-operate procedure on a single paper-based device to extract exosomal nucleic acid biomarkers for downstream analysis. Exosomes from biological samples were captured by anti-CD63-modified papers before being intermittently lysed by high-temperature, short-time treatment with double-distilled water to release their internal components. Exosomal nucleic acids were finally adsorbed by sol-gel silica for downstream analysis. Empirical trials not only revealed that sporadically dropping 95 °C ddH

Indexed as

ExosomesMicroRNAsPaperTetraspanin 30Biomarkers, TumorHumansPoint-of-Care TestingBiomarkers, TumorCD63 protein, humanMicroRNAsMIRN21 microRNA, humanTetraspanin 30Chronic woundExosomal biomarkerExosomal nucleic acid extractionHuman plasmaIntermittent lysisPaper-based deviceSilica nanoparticles

Identifiers

PMID39093424

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.