Molecular basis for sortase-catalyzed pilus tip assembly.

Aadil H Bhat, Chungyu Chang, Asis Das, Hung Ton-That

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Article in mBio, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

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4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Aadil H BhatDivision of Oral & Systemic Health Sciences, School of Dentistry, University of California, Los Angeles, California, USA.

Chungyu ChangDivision of Oral & Systemic Health Sciences, School of Dentistry, University of California, Los Angeles, California, USA.ORCID 0000-0001-6127-3086

Asis DasDepartment of Medicine, Neag Comprehensive Cancer Center, School of Medicine, University of Connecticut Health Center, Farmington, Connecticut, USA.

Hung Ton-ThatDivision of Oral & Systemic Health Sciences, School of Dentistry, University of California, Los Angeles, California, USA.ORCID 0000-0003-1611-0469

Funding

Molecular Assembly on the Cell Surface of ActinomycesR01DE017382 · NIDCR · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI Hung Ton-That · 2008 to 2026

$6.7M

NIDCR NIH HHS R01 DE017382

6 · The paper itself

Abstract

During pilus assembly within the Gram-positive bacterial envelope, membrane-bound sortase enzymes sequentially crosslink specific pilus protein monomers through their cell wall sorting signals (CWSS), starting with a designated tip pilin, followed by the shaft made of another pilin, ultimately anchoring the fiber base pilin to the cell wall. To date, the molecular determinants that govern pilus tip assembly and the underlying mechanism remain unknown. Here, we addressed this in the model organism IMPORTANCE: Gram-positive pili, whose precursors harbor a cell wall sorting signal (CWSS) needed for sortase-mediated pilus assembly, typically comprise a pilus shaft and a tip adhesin. How a pilin becomes a pilus tip, nevertheless, remains undetermined. We demonstrate here in

Indexed as

ActinomycesBacterial ProteinsCysteine EndopeptidasesFimbriae, BacterialFimbriae ProteinsAminoacyltransferasesCell WallProtein Sorting SignalsAminoacyltransferasesBacterial ProteinsCysteine EndopeptidasesFimbriae ProteinsProtein Sorting SignalsActinomyces oriscell wall anchoringcoaggregationpilus assemblysecretionsortasetip pilin

Identifiers

PMID39092925

DOI10.1128/mbio.01484-24

PMCPMC11389406

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.