ArticleRecent advances in food, nutrition & agriculture2025
Quantification using a HPLC-FLD-based Detector and Molecular Identification of Ochratoxin-A Producing
Article in Recent advances in food, nutrition & agriculture, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
aimQuantification using an HPLC-FLD based detector and Molecular identification of Ochratoxin-A producing Aspergillus Species isolated from stored grain samples.
backgroundFungi are cosmopolitan in origin and are known to grow in any suitable substratum. In the present investigation, Aspergillus species isolated from stored grain samples were analyzed for ochratoxin-A production.
objectiveThe objective of this study is the quantification of Ochratoxin and identification of ochratoxigenic fungi.
methodsA total of n=34 black Aspergilli and n=1 Ochre Aspergilli were isolated from grain samples of Bihar, India and it was tested for OTA production. The limit of detection (LOD) is found to be 0.33 μg/kg and the limit of quantification (LOQ) is found to be 1 μg/kg for OTA in HPLC-FLD.
resultsIn the present study, out of all the fungal isolates, only TiB fungal isolate was able to produce the ochratoxin-A above the level of LOQ. The positive isolate TiB obtained from stored sesame seed samples was able to produce 25.54 μg/kg of OTA. ITS sequence analysis of TiB isolate was able to match 100% with Aspergillus welwitschiae and Aspergillus foetidus.
conclusionThis is the initial report of ochratoxigenic Aspergillus fungal isolate isolated from stored sesame seed samples of flood-prone areas of Bihar, India.
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