Evidence map›Paper›PMID 39091738›Full record

ArticlebioRxiv : the preprint server for biology2024

Limiting the impact of protein leakage in single-cell proteomics.

Andrew Leduc, Yanxin Xu, Gergana Shipkovenska, Zhixun Dou, Nikolai Slavov

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Andrew LeducDepartments of Bioengineering, Biology, Chemistry and Chemical Biology, Single Cell Proteomics Center, Northeastern University, Boston, MA 02115, USA.ORCID 0000-0001-6234-0623
Yanxin XuCenter for Regenerative Medicine, Massachusetts General Hospital, Boston, MA, USA.
Gergana ShipkovenskaCenter for Regenerative Medicine, Massachusetts General Hospital, Boston, MA, USA.
Zhixun DouCenter for Regenerative Medicine, Massachusetts General Hospital, Boston, MA, USA.
Nikolai SlavovDepartments of Bioengineering, Biology, Chemistry and Chemical Biology, Single Cell Proteomics Center, Northeastern University, Boston, MA 02115, USA.ORCID 0000-0003-2035-1820

Funding

Single-cell proteomic identification of novel markers of senescenceUH3CA268117 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI DOU, ZHIXUN, RAJAGOPAL, JAYARAJ · 2023 to 2024
$1.9M
Molecular and metabolic influences on the activation of monocytes and macrophages at single-cell resolutionR35GM148218 · NIGMS · NORTHEASTERN UNIVERSITY · PI Nikolai Slavov · 2023 to 2026
$1.5M
Collaborative Research: DMS/NIGMS 2: Methods for Systematic Analysis of Post-transcriptional Regulation in Single CellsR01GM144967 · NIGMS · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI FRANKS, ALEXANDER, SLAVOV, NIKOLAI · 2021 to 2024
$779k
NCI NIH HHS UH3 CA268117NIGMS NIH HHS R01 GM144967NIGMS NIH HHS R35 GM148218
6 · The paper itself

Abstract

Limiting artifacts during sample preparation can significantly increase data quality in single-cell proteomics experiments. Towards this goal, we characterize the impact of protein leakage by analyzing thousands of primary single cells that were prepared either fresh immediately after dissociation or cryopreserved and prepared at a later date. We directly identify permeabilized cells and use the data to define a signature for protein leakage. We use this signature to build a classifier for identifying damaged cells that performs accurately across cell types and species.

Identifiers

PMID39091738
PMCPMC11291177

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.