ArticleFrontiers in veterinary science2024
Development and optimization of sampling techniques for environmental samples from African swine fever virus-contaminated surfaces with no organic contaminants.
Article in Frontiers in veterinary science, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Methods for detecting highly pathogenic avian influenza H5N1 virus in dairy processing environments.Frontiers in microbiology · 2026Article
- Effect of temperature, relative humidity, and time on the detection of swine RNA viruses (PRRSV, PEDV, IAV) inoculated onto filter papers.Frontiers in cellular and infection microbiology · 2026Article
- Surveillance of Antimicrobial Resistance in the Asian Seabass (Foods (Basel, Switzerland) · 2025Article
- The effect of freeze-thaw and storage on African swine fever virus detection in environmental samples.Frontiers in veterinary science · 2025Article
- Comparison of the recovery of PCR-detectable porcine epidemic diarrhea virus and porcine reproductive and respiratory syndrome virus from filter papers under laboratory conditions.Frontiers in cellular and infection microbiology · 2025Article
- Comparison of DNA extraction methods for detecting African swine fever virus in feed and environmental samples.Frontiers in veterinary science · 2025Article
- Improved African Swine Fever Detection for Environmental Samples in the Presence of Organic Contaminants.Transboundary and emerging diseases · 2024Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
African swine fever (ASF) is a highly contagious diseases in domestic pigs and wild boars with up to 100% mortality. ASF virus (ASFV) is a causative agent responsible for ASF and highly resistant in environments, which creates a significant challenge for the control and eradication of the virus. Despite the geographical expansion of ASFV and international movement of products to sustain the swine production system, there is limited knowledge on the use of environmental samples to perform surveillance to prevent the introduction of ASFV into ASFV-free areas and for control of transmission in affected areas. Therefore, this study aimed to develop and optimize sampling techniques for environmental samples for ASFV detection. The stainless steel surfaces were contaminated with ASFV-infected blood, swabbed using different devices, and then processed through different techniques. The environmental samples were processed and tested using qPCR analysis. The results showed that the use of pre-moistened gauze surgical sponges, sweeping pads, and sponge sticks resulted in increased sensitivity, when compared to either dry sampling devices or Dacron swab. In particular, the combination of the sponge stick and the commercial nucleic acid preservative supported the best detection of ASFV DNA on the clean stainless steel surfaces evaluated. Pre-incubation for the short period of time and centrifugation at low speed were sufficient to provide satisfactory diagnostic sensitivity of ASFV detection using qPCR for environmental samples. Our findings contribute to the development of techniques for environmental samples for ASFV surveillance to prevent the introduction and dissemination of ASFV.
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