ArticleNature chemical biology2025
Nanometer-resolution tracking of single cargo reveals dynein motor mechanisms.
Article in Nature chemical biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Spontaneous and indefinite blinking in upconverting nanoparticles for ångström-precision multicolour super-resolution imaging.Nature nanotechnology · 2026Article
- Characterizing dynamics of productive dynein stepping by MINFLUX.Nature structural & molecular biology · 2026Article
- Upconverting nanoparticles enable bioimaging with sub-ångström precision.Nature nanotechnology · 2026Article
- ErbB family receptor dimerization dynamics and dysregulation via long-term single-molecule imaging.Cell · 2026Article
- Mitochondrial dynamics in neurodevelopment and neurodevelopmental disorders.Nature reviews. Neuroscience · 2026Review
- Enhanced Single-Particle Upconversion Imaging via Energy Migration Boosting.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Sub-10 nm upconversion nanocrystals for long-term single-particle tracking.Nature communications · 2025Article
- On The Retrograde Transport of RNA-Loaded Lipid Nanoparticles Designed for Brain Delivery.ACS nanoscience Au · 2025Article
- Visualising sub-second dynamics of nanoparticle extravasationbioRxiv : the preprint server for biology · 2025Article
- The mechanochemical cycle of reactive full-length human dynein 1.Nature structural & molecular biology · 2025Article
- Sidelobe-free deterministic 3D nanoscopy with λ/33 axial resolution.Light, science & applications · 2025Article
- Multiple kinesins speed up cargo transport in crowded environments by sharing load.Communications biology · 2025Article
- MINFLUX reveals dynein stepping in live neurons.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Stepping dynamics of dynein characterized by MINFLUX.bioRxiv : the preprint server for biology · 2024Article
- Recent Advances in Single-Molecule Tracking and Imaging Techniques.Annual review of analytical chemistry (Palo Alto, Calif.) · 2023Review
- Direct observation of motor protein stepping in living cells using MINFLUX.Science (New York, N.Y.) · 2023Article
- Japan-US symposium on motor proteins and associated single-molecule biophysics.Biophysics and physicobiology · 2022Article
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Authors and funding
8 authors.
Funding
Abstract
Cytoplasmic dynein is essential for intracellular transport. Despite extensive in vitro characterizations, how the dynein motors transport vesicles by processive steps in live cells remains unclear. To dissect the molecular mechanisms of dynein, we develop optical probes that enable long-term single-particle tracking in live cells with high spatiotemporal resolution. We find that the number of active dynein motors transporting cargo switches stochastically between one and five dynein motors during long-range transport in neuronal axons. Our very bright optical probes allow the observation of individual molecular steps. Strikingly, these measurements reveal that the dwell times between steps are controlled by two temperature-dependent rate constants in which two ATP molecules are hydrolyzed sequentially during each dynein step. Thus, our observations uncover a previously unknown chemomechanical cycle of dynein-mediated cargo transport in living cells.
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