Evidence map›Paper›PMID 39085294›Full record

ArticleScientific reports2024

Sodium Tanshinone IIA Sulfonate alleviates vascular senescence in diabetic mice by modulating the A20-NFκB-NLRP3 inflammasome-catalase pathway.

Wei Wei, Yan-Yan Heng, Fei-Fei Wu, Hao-Yu Dong, Peng-Fei Zhang, Jing-Xia Li, Chun-Yan Liu, Bing-Jie Yang, Jia-Ning Fu, Xin-Yue Liang

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In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Wei WeiDepartment of Pharmacology, Changzhi Medical College, No.161, Jiefang East Street, Changzhi, 046000, Shanxi, China. jaywei@czmc.edu.cn.
Yan-Yan HengDepartment of Nephrology Heping Hospital, Affiliated to Changzhi Medical College, No.110, Yanan Road South, Changzhi, Shanxi, China.
Fei-Fei WuDepartment of Endocrinology and Institute of Endocrinology and Metabolic Disease, Heping Hospital Affiliated to Changzhi Medical College, No.110, Yanan Road South, Changzhi, 046000, Shanxi, China.
Hao-Yu DongDepartment of Endocrinology and Institute of Endocrinology and Metabolic Disease, Heping Hospital Affiliated to Changzhi Medical College, No.110, Yanan Road South, Changzhi, 046000, Shanxi, China.
Peng-Fei ZhangDepartment of Nephrology Heping Hospital, Affiliated to Changzhi Medical College, No.110, Yanan Road South, Changzhi, Shanxi, China.
Jing-Xia LiDepartment of Anesthesia, Changzhi Medical College, No.161, Jiefang East Street, Changzhi, Shanxi, China.
Chun-Yan LiuDepartment of Anesthesia, Changzhi Medical College, No.161, Jiefang East Street, Changzhi, Shanxi, China.
Bing-Jie YangDepartment of Stomatology, Changzhi Medical College, No.161, Jiefang East Street, Changzhi, Shanxi, China.
Jia-Ning FuDepartment of Stomatology, Changzhi Medical College, No.161, Jiefang East Street, Changzhi, Shanxi, China.
Xin-Yue LiangDepartment of Medical Imageology, Changzhi Medical College, No.161, Jiefang East Street, Changzhi, Shanxi, China.

Funding

Changzhi Medical College BS202115Department of Science and Technology of Shanxi 202203021211107Health Commission of Shanxi Province 2022021Heping Hospital Affiliated to Changzhi Medical College HPYJ201921Heping Hospital Affiliated to Changzhi Medical College HPYJ202218Scientific and Technological Innovation Programs (STIP) of Higher Education Institutions in Shanxi 2021L351
6 · The paper itself

Abstract

Diabetes accelerates vascular senescence, which is the basis for atherosclerosis and stiffness. The activation of NOD-like receptor family pyrin domain containing 3 (NLRP3) inflammasome and oxidative stress are closely associated with the deteriorative senescence in endothelial cells (ECs) and vascular smooth muscle cells (VSMCs). For decades, Sodium Tanshinone IIA Sulfonate (STS) has been utilized as a cardiovascular medicine with acknowledged anti-inflammatory and anti-oxidative properties. Nevertheless, the impact of STS on vascular senescence remains unexplored in diabetes. Diabetic mice, primary ECs and VSMCs were transfected with the NLRP3 overexpression/knockout plasmid, the tumor necrosis factor alpha-induced protein 3 (TNFAIP3/A20) overexpression/knockout plasmid, and treated with STS to detect senescence-associated markers. In diabetic mice, STS treatment maintained catalase (CAT) level and vascular relaxation, reduced hydrogen peroxide probe (ROSgreen) fluorescence, p21 immunofluorescence, Senescence β-Galactosidase Staining (SA-β-gal) staining area, and collagen deposition in aortas. Mechanistically, STS inhibited NLRP3 phosphorylation (serine 194), NLRP3 dimer formation, NLRP3 expression, and NLRP3-PYCARD (ASC) colocalization. It also suppressed the phosphorylation of IkappaB alpha (IκBα) and NFκB, preserved A20 and CAT levels, reduced ROSgreen density, and decreased the expression of p21 and SA-β-gal staining in ECs and VSMCs under HG culture. Our findings indicate that STS mitigates vascular senescence by modulating the A20-NFκB-NLRP3 inflammasome-CAT pathway in hyperglycemia conditions, offering novel insights into NLRP3 inflammasome activation and ECs and VSMCs senescence under HG culture. This study highlights the potential mechanism of STS in alleviating senescence in diabetic blood vessels, and provides essential evidence for its future clinical application.

Indexed as

Cellular SenescenceDiabetes Mellitus, ExperimentalInflammasomesNF-kappa BNLR Family, Pyrin Domain-Containing 3 ProteinPhenanthrenesSignal TransductionAnimalsCatalaseEndothelial CellsMaleMiceMice, Inbred C57BLMuscle, Smooth, VascularMyocytes, Smooth MuscleCatalaseInflammasomesNF-kappa BNLR Family, Pyrin Domain-Containing 3 ProteinNlrp3 protein, mousePhenanthrenestanshinone II A sodium sulfonateCatalaseDiabetic vascular diseaseEndothelial cellsNLRP3 inflammasomeSenescenceVascular smooth muscle cells

Identifiers

PMID39085294
PMCPMC11291694

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.