ArticlePloS one2024
Advancing micro-electrometric techniques for the detection of organophosphate and carbamate residues using cricket cholinesterase.
Article in PloS one, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Fluorometric and Colorimetric Biosensors for the Assay of Cholinesterase Inhibitors.Sensors (Basel, Switzerland) · 2025Review
- Portable Thiocholine-Based Sensor for Monitoring Blood Cholinesterase Activity and Detecting Organophosphate and Carbamate Pesticides Using Personal Glucose Meters.Foods (Basel, Switzerland) · 2025Article
- Micro-Electrometric Method for Assessing Cholinesterase Activity in Human Whole Blood.Methods and protocols · 2025Article
- Whole blood cholinesterase activity in humans and ruminants: Comparison using a modified electrometric method.Open veterinary journal · 2025Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The widespread use of organophosphate (OP) and carbamate (CM) pesticides requires efficient and cost-effective detection methods. This study introduces a micro-electrometric method using cricket cholinesterase (ChE) to detect OP and CM residues, providing a rapid and economical alternative to conventional chromatographic techniques. The parameters of the method, including the substrate concentration, incubation temperature, and incubation time, were optimized. By leveraging the sensitivity of cricket ChE to OP and CM inhibition, this approach translates enzyme inhibition into an electrical signal to quantify pesticide levels, achieving an impressive limit of detection (LOD) from 0.036 to 0.086 parts per million (ppm). This method demonstrated reproducibility and stability, making it suitable for field applications and on-site testing across various environmental matrices. This research represents a significant advancement in pesticide residue analysis with potential applications in the development of portable biosensor devices for real-time environmental monitoring and public health protection.
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Registered trials
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