Evidence map›Paper›PMID 39083080›Full record

ArticleCurrent genetics2024

Optimizing CaCl

Damanjeet Kaur, Vijay Singh, Saurabh Gupta

Abstract read
PubMed Publisher
In one paragraph

Article in Current genetics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Damanjeet KaurDepartment of Microbiology, Mata Gujri College, Fatehgarh Sahib, Punjab, 140406, India.
Vijay SinghDepartment of Botany, Mata Gujri College, Fatehgarh Sahib, Punjab, 140406, India.
Saurabh GuptaDepartment of Microbiology, Mata Gujri College, Fatehgarh Sahib, Punjab, 140406, India. sau27282@gmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cloning and expression of a gene in the desired host is required for optimum production in recombinant strains. The present research is the first attempt to optimize the physiological conditions for the transformation of Pseudomonas aeruginosa SDK-6 with pJN105. Different factors, such as inoculum size, incubation period, heat shock temperature, and heat shock time were optimized using one factor at a time (OFAT) followed by the selection of transformants using gentamicin resistance marker. The maximum number of transformants (2.002 ± 0.077 × 10

Indexed as

Cloning, MolecularGenetic VectorsPlasmidsPseudomonas aeruginosaTransformation, BacterialCalcium ChlorideCalcium ChlorideHeat shockOFATpJN105Pseudomonas aeruginosaTransformants

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.