Evidence map›Paper›PMID 39081856›Full record

ArticleBiodesign research2024

A Light-Driven In Vitro Enzymatic Biosystem for the Synthesis of α-Farnesene from Methanol.

Xinyue Gui, Fei Li, Xinyu Cui, Ranran Wu, Dingyu Liu, Chunling Ma, Lijuan Ma, Huifeng Jiang, Chun You, Zhiguang Zhu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biodesign research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Xinyue GuiKey Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, The College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.
Fei LiKey Laboratory of Engineering Biology for Low-Carbon Manufacturing, TianjinInstitute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
Xinyu CuiKey Laboratory of Engineering Biology for Low-Carbon Manufacturing, TianjinInstitute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
Ranran WuKey Laboratory of Engineering Biology for Low-Carbon Manufacturing, TianjinInstitute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
Dingyu LiuKey Laboratory of Engineering Biology for Low-Carbon Manufacturing, TianjinInstitute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
Chunling MaKey Laboratory of Engineering Biology for Low-Carbon Manufacturing, TianjinInstitute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
Lijuan MaKey Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, The College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.
Huifeng JiangKey Laboratory of Engineering Biology for Low-Carbon Manufacturing, TianjinInstitute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
Chun YouUniversity of Chinese Academy of Sciences, Beijing 100049, China.
Zhiguang ZhuKey Laboratory of Engineering Biology for Low-Carbon Manufacturing, TianjinInstitute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.ORCID https://orcid.org/0000-0002-6625-5087

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Terpenoids of substantial industrial interest are mainly obtained through direct extraction from plant sources. Recently, microbial cell factories or in vitro enzymatic biosystems have emerged as promising alternatives for terpenoid production. Here, we report a route for the synthesis of α-farnesene based on an in vitro enzyme cascade reaction using methanol as an inexpensive and renewable C1 substrate. Thirteen biocatalytic reactions divided into 2 modules were optimized and coupled to achieve methanol-to-α-farnesene conversion via integration with natural thylakoid membranes as a green energy engine. This in vitro enzymatic biosystem driven by light enabled the production of 1.43 and 2.40 mg liter

Identifiers

PMID39081856
PMCPMC11286291

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.