Evidence map›Paper›PMID 39081363›Full record

ArticleISME communications2024

Air monitoring by nanopore sequencing.

Tim Reska, Sofya Pozdniakova, Sílvia Borràs, Albert Perlas, Ela Sauerborn, Lídia Cañas, Michael Schloter, Xavier Rodó, Yuanyuan Wang, Barbro Winkler and 2 more

Abstract read
In one paragraph

Article in ISME communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. The Indoor Microbiome: Sampling, Analysis and Emerging Trends.Environmental microbiology reports · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Tim ReskaHelmholtz AI, Helmholtz Zentrum München, 85764 Neuherberg, Germany.
Sofya PozdniakovaAIRLAB, Climate and Health (CLIMA) group, ISGlobal, 08003 Barcelona, Spain.
Sílvia BorràsAIRLAB, Climate and Health (CLIMA) group, ISGlobal, 08003 Barcelona, Spain.
Albert PerlasHelmholtz AI, Helmholtz Zentrum München, 85764 Neuherberg, Germany.
Ela SauerbornHelmholtz AI, Helmholtz Zentrum München, 85764 Neuherberg, Germany.
Lídia CañasAIRLAB, Climate and Health (CLIMA) group, ISGlobal, 08003 Barcelona, Spain.
Michael SchloterTechnical University of Munich, School of Life Sciences, 85354 Freising, Germany.
Xavier RodóAIRLAB, Climate and Health (CLIMA) group, ISGlobal, 08003 Barcelona, Spain.
Yuanyuan WangTechnical University of Munich, School of Engineering and Design, 80333 Munich, Germany.
Barbro WinklerResearch Unit Environmental Simulation (EUS), Helmholtz Zentrum München, 85764 Neuherberg, Germany.
Jörg-Peter SchnitzlerResearch Unit Environmental Simulation (EUS), Helmholtz Zentrum München, 85764 Neuherberg, Germany.
Lara UrbanHelmholtz AI, Helmholtz Zentrum München, 85764 Neuherberg, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

While the air microbiome and its diversity are essential for human health and ecosystem resilience, comprehensive air microbial diversity monitoring has remained rare, so that little is known about the air microbiome's composition, distribution, or functionality. Here we show that nanopore sequencing-based metagenomics can robustly assess the air microbiome in combination with active air sampling through liquid impingement and tailored computational analysis. We provide fast and portable laboratory and computational approaches for air microbiome profiling, which we leverage to robustly assess the taxonomic composition of the core air microbiome of a controlled greenhouse environment and of a natural outdoor environment. We show that long-read sequencing can resolve species-level annotations and specific ecosystem functions through de novo metagenomic assemblies despite the low amount of fragmented DNA used as an input for nanopore sequencing. We then apply our pipeline to assess the diversity and variability of an urban air microbiome, using Barcelona, Spain, as an example; this randomized experiment gives first insights into the presence of highly stable location-specific air microbiomes within the city's boundaries, and showcases the robust microbial assessments that can be achieved through automatable, fast, and portable nanopore sequencing technology.

Indexed as

antimicrobial resistancebioaerosolsde novo assemblyinfectious diseaselong-read sequencingmetagenomicsnanopore sequencingshotgun sequencingurban air microbiome

Identifiers

PMID39081363
PMCPMC11287864

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.