Evidence map›Paper›PMID 39080808›Full record

ArticleBiology of sex differences2024

Smad4 and FoxH1 potentially interact to regulate cyp19a1a promoter in the ovary of ricefield eel (Monopterus albus).

Qiqi Chen, Deying Yang, Mingqiang Chen, Jinxin Xiong, Junjie Huang, Wenxiang Ding, Kuo Gao, Bolin Lai, Li Zheng, Ziting Tang and 3 more

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Article in Biology of sex differences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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1 · What the graph read from it

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3 · Its place in the literature

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1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

13 authors.

Qiqi Chen *College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Deying Yang *College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Mingqiang Chen *College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Jinxin XiongCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Junjie HuangCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Wenxiang DingCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Kuo GaoCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Bolin LaiCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Li ZhengCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Ziting TangCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Mingwang ZhangCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Taiming YanCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Zhi HeCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China. zhihe@sicau.edu.cn.ORCID 0000-0001-9164-2372

Funding

National Natural Science Foundation of China 31972777
6 · The paper itself

Abstract

backgroundCyp19a1a is a key enzyme in the pathway that converts androgens into estrogen and is regulated by TGF-β signaling. Smad4 and FoxH1 are downstream effectors of TGF-β signaling and may play important roles in ovarian development in M. albus.

methodsWe investigated the expression pattern of the Smad4 and FoxH1 using qRT‒PCR and immunofluorescence, then tested the changes of smad4 and foxh1 by qRT‒PCR after ovary incubation with FSH in vitro, and analysed the regulation of cyp19a1a transcription by Smad4 and FoxH1 by dual-luciferase reporter assays.

resultsWe found that Smad4 encoded a putative protein of 449 amino acids and harbored the three conserved domains typical of this protein family. Smad4 and foxh1 exhibited similar expression patterns during ovarian development and after FSH incubation, with Pearson's coefficients of 0.873 and 0.63-0.81, respectively. Furthermore, Smad4, FoxH1 and Cyp19a1a colocalized in the granulosa cells and theca cells of ovaries during the mid-to-late vitellogenic stage. Smad4 repressed cyp19a1a activity via SBE1 (- 1372/-1364) and SBE2 (- 415/-407) in the cyp19a1a promoter, whereas mutating SBE1 or SBE2 restored cyp19a1a promoter activity. Co-overexpression of Smad4 and FoxH1 significantly reduced cyp19a1a promoter activity.

conclusionsThis study provides new insights into the potential functions of transcription factors Smad4 and FoxH1 in ovarian development and the transcriptional regulation mechanism of cyp19a1a in M. albus, which will reveal Smad4/FoxH1-mediated TGF-β signaling in reproduction and the regulation of the cyp19a1a. Aromatase, encoded by cyp19a1a, is involved in ovarian development and plays an important role in the quality of eggs, as well the sex ratio, of the teleost fish, M. albus. The research on the transcriptional regulation of cyp19a1a has contributed to the understanding of its role in ovarian development. In previous study, it was shown that FoxH1 inhibits cyp19a1a transcription. In the present study, Smad4 was confirmed as a cyp19a1a transcriptional repressor and Smad4 may also coordinate with FoxH1 to repress cyp19a1a transcription. At present, we provide a new perspective for the transcriptional regulation of cyp19a1a by transcription factors Smad4 and FoxH1 in teleost fish ovary. In the future, the regulatory networks of Smad4 and FoxH1 will be further studied and the gene editing technology will be applied to screen specific regulatory factors of cyp191a1a gene, so as to alter the female cycle and modulate the sex ratio of the eggs production.

Indexed as

AromataseEelsForkhead Transcription FactorsOvaryPromoter Regions, GeneticSmad4 ProteinAnimalsFemaleFish ProteinsFollicle Stimulating HormoneAromataseFish ProteinsFollicle Stimulating HormoneForkhead Transcription FactorsSmad4 ProteinCyp19a1a promoterFoxH1Monopterus albusOvary developmentSmad4

Identifiers

PMID39080808
PMCPMC11290265

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.