Evidence map›Paper›PMID 39078114›Full record

ArticleBioinformatics (Oxford, England)2024

AssemblyQC: a Nextflow pipeline for reproducible reporting of assembly quality.

Usman Rashid, Chen Wu, Jason Shiller, Ken Smith, Ross Crowhurst, Marcus Davy, Ting-Hsuan Chen, Ignacio Carvajal, Sarah Bailey, Susan Thomson and 1 more

Abstract read
In one paragraph

Article in Bioinformatics (Oxford, England), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Review
  7. Article
  8. Article
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Usman RashidMolecular & Digital Breeding, The New Zealand Institute for Plant and Food Research Limited, 1025 Auckland, New Zealand.
Chen WuMolecular & Digital Breeding, The New Zealand Institute for Plant and Food Research Limited, 1025 Auckland, New Zealand.
Jason ShillerMolecular & Digital Breeding, The New Zealand Institute for Plant and Food Research Limited, 3182 Te Puke, New Zealand.
Ken SmithMolecular & Digital Breeding, The New Zealand Institute for Plant and Food Research Limited, 1025 Auckland, New Zealand.
Ross CrowhurstMolecular & Digital Breeding, The New Zealand Institute for Plant and Food Research Limited, 1025 Auckland, New Zealand.
Marcus DavyMolecular & Digital Breeding, The New Zealand Institute for Plant and Food Research Limited, 3182 Te Puke, New Zealand.
Ting-Hsuan ChenMolecular & Digital Breeding, The New Zealand Institute for Plant and Food Research Limited, 7608 Lincoln, New Zealand.
Ignacio CarvajalMolecular & Digital Breeding, The New Zealand Institute for Plant and Food Research Limited, 1025 Auckland, New Zealand.
Sarah BaileyMolecular & Digital Breeding, The New Zealand Institute for Plant and Food Research Limited, 1025 Auckland, New Zealand.
Susan ThomsonMolecular & Digital Breeding, The New Zealand Institute for Plant and Food Research Limited, 7608 Lincoln, New Zealand.
Cecilia H DengMolecular & Digital Breeding, The New Zealand Institute for Plant and Food Research Limited, 1025 Auckland, New Zealand.ORCID 0000-0002-2954-762X

Funding

Traits for Life Indoors
6 · The paper itself

Abstract

summaryGenome assembly projects have grown exponentially due to breakthroughs in sequencing technologies and assembly algorithms. Evaluating the quality of genome assemblies is critical to ensure the reliability of downstream analysis and interpretation. To fulfil this task, we have developed the AssemblyQC pipeline that performs file-format validation, contaminant checking, contiguity measurement, gene- and repeat-space completeness quantification, telomere inspection, taxonomic assignment, synteny alignment, scaffold examination through Hi-C contact-map visualization, and assessments of completeness, consensus quality and phasing through k-mer analysis. It produces a comprehensive HTML report with method descriptions, tables, and visualizations. AVAILABILITY AND IMPLEMENTATION: The pipeline uses Nextflow for workflow orchestration and adheres to the best-practice established by the nf-core community. This pipeline offers a reproducible, scalable, and portable method to assess the quality of genome assemblies-the code is available online at GitHub: https://github.com/Plant-Food-Research-Open/assemblyqc.

Indexed as

SoftwareAlgorithmsGenomeGenomicsHigh-Throughput Nucleotide SequencingSequence Analysis, DNA

Identifiers

PMID39078114
PMCPMC11333564

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.