Evidence map›Paper›PMID 39073302›Full record

ArticleChemistry & biodiversity2024

Bioactive Alkaloids from Nepalese Corydalis chaerophylla D.C. Acting on the Regulation of PCSK9 and LDL-R In Vitro.

Binita Maharjan, Ilaria Rossi, Stefania Sut, Timila Shrestha, Lok Kumar Shrestha, Jonathan P Hill, Katsuhiko Ariga, Veronica Benetazzo, Maria Pia Adorni, Bianca Papotti and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chemistry & biodiversity, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. LC-DAD-MSChemistry & biodiversity · 2025
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Binita MaharjanDepartment of Chemistry, Amrit Campus, Tribhuvan University, Kathmandu, 44600, Nepal.
Ilaria RossiDepartment of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova, Italy.
Stefania SutDepartment of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova, Italy.
Timila ShresthaDepartment of Chemistry, Amrit Campus, Tribhuvan University, Kathmandu, 44600, Nepal.
Lok Kumar ShresthaResearch Center for Materials Nano Architectonics (MANA), National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba, Ibaraki, 305-0044, Japan.
Jonathan P HillResearch Center for Materials Nano Architectonics (MANA), National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba, Ibaraki, 305-0044, Japan.
Katsuhiko ArigaResearch Center for Materials Nano Architectonics (MANA), National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba, Ibaraki, 305-0044, Japan.
Veronica BenetazzoDepartment of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova, Italy.
Maria Pia AdorniDepartment of Medicine and Surgery, University of Parma, Parma, Italy.
Bianca PapottiDepartment of Food and Drug, University of Parma, Parma, Italy.
Shyam Sharan ShresthaHimalayan Research and Development Center Nepal, Kathmandu, Nepal.
Ram Lal Swagat ShresthaDepartment of Chemistry, Amrit Campus, Tribhuvan University, Kathmandu, 44600, Nepal.
Nicola FerriDepartment of Medicine, University of Padova, Padova, Italy.
Stefano Dall'AcquaDepartment of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova, Italy.

Funding

University Grants Commission (UGC) PhD-75/76-S&T-6
6 · The paper itself

Abstract

Four new alkaloids Chaeronepaline-A (1), Chaeronepaline-B (2), Chaeronepaline-C (3), and Chaeronepaline-D (4) were isolated from Corydalis chaerophylla D.C. collected from Nepal and their structures were elucidated by spectroscopic data, 1D, 2D NMR and mass spectrometry. The structures were established as 3,12- Dimethoxy-5,6-dihydroisoquinolino [2,1-b] isoquinolin- 7- ium- 2, 9- diol (1), 7-Methyl-2,3 : 11,12-bis(methylenedioxy)-7,13a-secoberbin-13-14-epoxide (2), 7- methyl-5, 6, 7, 8- tetrahydro- 8H-spiro-9,14-dihydroxy-11,12-methylenedioxy-indane-isoquinoline (3) and 7- methyl-5, 6, 7, 8- tetrahydro- 8H-spiro-9,14-dihydroxy-11,12-methylenedioxy-indane-isoquinoline-N-oxide (4). The new alkaloids were tested in human hepatoma cell line to assess their ability to modulate the expression of low-density lipoprotein receptor (LDL-R), of proprotein convertase subtilisin/kexin 9 (PCSK9) and to affect cellular cholesterol biosynthesis with the aim to evaluate their potential hypocholesterolemic effect. Results indicated that compounds 2 and 3 upregulate the LDLR, and inhibited the cholesterol biosynthesis with compound 2, which also reduced the secretion of PCSK9 by Huh7 cells. These in vitro data indicated a potential hypocholesterolemic effect of compound 2 that requires further in vivo validation.

Indexed as

AlkaloidsCorydalisProprotein Convertase 9Receptors, LDLCell Line, TumorDose-Response Relationship, DrugHep G2 CellsHeterocyclic Compounds, 4 or More RingsHumansMolecular ConformationMolecular StructureNepalSpiro CompoundsStructure-Activity RelationshipAlkaloidsHeterocyclic Compounds, 4 or More RingsPCSK9 protein, humanProprotein Convertase 9Receptors, LDLSpiro CompoundsCorydalis chaerophylla D.C.Isoquinoline alkaloidsLDL−RPCSK9

Identifiers

PMID39073302
PMCPMC11644109

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.