Evidence map›Paper›PMID 39072316›Full record

ArticleFrontiers in immunology2024

Flow cytometry-based diagnostic approach for inborn errors of immunity: experience from Algeria.

Azzeddine Tahiat, Reda Belbouab, Abdelghani Yagoubi, Saliha Hakem, Faiza Fernini, Malika Keddari, Hayet Belhadj, Souad Touri, Samira Aggoune, Jennifer Stoddard and 47 more

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Article in Frontiers in immunology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

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0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

57 authors.

Azzeddine TahiatDepartment of Medical Biology, Rouiba Hospital, University of Algiers 1, Algiers, Algeria.
Reda BelbouabDepartment of Pediatrics, Mustapha University Hospital, University of Algiers 1, Algiers, Algeria.
Abdelghani YagoubiPediatric Gastroenterology, Centre Algérois de Pédiatrie, Algiers, Algeria.
Saliha HakemDepartment of Pediatrics, Mustapha University Hospital, University of Algiers 1, Algiers, Algeria.
Faiza FerniniDepartment of Pediatrics, Mustapha University Hospital, University of Algiers 1, Algiers, Algeria.
Malika KeddariDepartment of Pediatrics, Mustapha University Hospital, University of Algiers 1, Algiers, Algeria.
Hayet BelhadjDepartment of Pediatrics, Central Hospital of the Army, Algiers, Algeria.
Souad TouriDepartment of Pediatrics, Blida University Hospital, University of Blida, Blida, Algeria.
Samira AggouneDepartment of Pediatrics, El-Harrach Hospital, University of Algiers 1, Algiers, Algeria.
Jennifer StoddardImmunology Service, Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, United States.
Julie NiemelaImmunology Service, Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, United States.
Farida ZerifiDepartment of Pediatrics, Ain Taya Hospital, University of Algiers 1, Algiers, Algeria.
Souhila MelziDepartment of Pediatrics, Bab El-Oued University Hospital, University of Algiers 1, Algiers, Algeria.
Rawda AbouraDepartment of Pediatrics, Bab El-Oued University Hospital, University of Algiers 1, Algiers, Algeria.
Amina Saad-DjaballahDepartment of Pediatrics, Bologhine Hospital, University of Algiers 1, Algiers, Algeria.
Yacine FerhaniDepartment of Pediatrics, Mustapha University Hospital, University of Algiers 1, Algiers, Algeria.
Abdalbasset KetfiDepartment of Pneumology, Rouiba Hospital, University of Algiers 1, Algiers, Algeria.
Hassen MessaoudiDepartment of Internal Medicine, Rouiba Hospital, University of Algiers 1, Algiers, Algeria.
Tahar Bencharif MadaniDepartment of Pediatrics, Mansourah Hospital, University of Constantine, Constantine, Algeria.
Zouleikha BenhacineDepartment of Pediatrics, Constantine University Hospital, University of Constantine, Constantine, Algeria.
Abdelhak DehimiDepartment of Pediatrics, Setif University Hospital, University of Setif, Setif, Algeria.
Kamelia OkkaDepartment of Pediatrics, Setif University Hospital, University of Setif, Setif, Algeria.
Fairouz AmrouneDepartment of Pediatrics, Setif University Hospital, University of Setif, Setif, Algeria.
Meriem FellahiDepartment of Pediatrics, Setif University Hospital, University of Setif, Setif, Algeria.
Chafa BendahmaneDepartment of Pediatrics, Meftah Hospital, Blida, Algeria.
Radia KhoulaniDepartment of Pediatrics, Meftah Hospital, Blida, Algeria.
Asma OukilDepartment of Medical Biology, Rouiba Hospital, University of Algiers 1, Algiers, Algeria.
Asma SoufaneDepartment of Medical Biology, Rouiba Hospital, University of Algiers 1, Algiers, Algeria.
Imene BourelafDepartment of Medical Biology, Rouiba Hospital, University of Algiers 1, Algiers, Algeria.
Chahynez BoubidiDepartment of Pediatrics A, Hussein Dey University Hospital, University of Algiers 1, Algiers, Algeria.
Nadia BoukhenfoufDepartment of Pediatrics, Rouiba Hospital, Algiers, Algeria.
Mohamed Amine IfriDepartment of Pediatrics, Thenia Hospital, Boumerdes, Algeria.
Noureddine KhelafiDepartment of Pediatrics, Thenia Hospital, Boumerdes, Algeria.
Houda BoudiafDepartment of Pediatric Oncology, Mustapha University Hospital, University of Algiers 1, Algiers, Algeria.
Tahar Khelifi TouhamiPrivate Practitioner, Constantine, Algeria.
Fethi MeçabihDepartment of Immunology, Institut Pasteur d'Algérie, University of Algiers 1, Algiers, Algeria.
Malika BoucelmaDepartment of Internal Medicine, Kouba Hospital, University of Algiers 1, Algiers, Algeria.
Amara ZelaciDepartment of Pediatrics, El Oued Hospital, El Oued, Algeria.
Ourida GacemDepartment of Pediatrics, Birtraria Hospital El Biar, University of Algiers 1, Algiers, Algeria.
Mohamed Samir LadjDepartment of Pediatrics, Birtraria Hospital El Biar, University of Algiers 1, Algiers, Algeria.
Azzedine MekkiDepartment of Pediatrics B, Hussein Dey University Hospital, University of Algiers 1, Algiers, Algeria.
Nadia BensaadiDepartment of Pediatrics, Tizi Ouzou University Hospital, University of Tizi Ouzou, Tizi Ouzou, Algeria.
Malika BenhalimaAlgiers Faculty of Pharmacy, University of Algiers 1, Algiers, Algeria.
Zoulikha ZeroualDepartment of Pediatrics A, Hussein Dey University Hospital, University of Algiers 1, Algiers, Algeria.
Belkacem BioudDepartment of Pediatrics, Setif University Hospital, University of Setif, Setif, Algeria.
Mustapha BenameurDepartment of Internal Medicine, Rouiba Hospital, University of Algiers 1, Algiers, Algeria.
Rachid BouhdjilaDepartment of Pediatrics, Constantine University Hospital, University of Constantine, Constantine, Algeria.
Zahir BouzerarDepartment of Pediatrics, Bab El-Oued University Hospital, University of Algiers 1, Algiers, Algeria.
Ouardia IbsaineDepartment of Pediatrics, Ain Taya Hospital, University of Algiers 1, Algiers, Algeria.
Hachemi MaoucheDepartment of Pediatrics, El-Harrach Hospital, University of Algiers 1, Algiers, Algeria.
Leila KedjiDepartment of Pediatrics, Blida University Hospital, University of Blida, Blida, Algeria.
Leila SmatiDepartment of Pediatrics, Bologhine Hospital, University of Algiers 1, Algiers, Algeria.
Rachida BoukariDepartment of Pediatrics, Mustapha University Hospital, University of Algiers 1, Algiers, Algeria.
Claude LambertCytometry Unit, Immunology Laboratory, Saint-Etienne University Hospital, Saint-Étienne, Lyon, France.
Sergio D RosenzweigImmunology Service, Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, United States.
Luigi D NotarangeloNational Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, United States.
Kamel DjenouhatDepartment of Medical Biology, Rouiba Hospital, University of Algiers 1, Algiers, Algeria.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Purpose: In this study, we retrospectively reviewed the use of flow cytometry (FCM) in the diagnosis of inborn errors of immunity (IEIs) at a single center in Algeria. Sharing insights into our practical experience, we present FCM based diagnostic approaches adapted to different clinical scenarios. Methods: Between May 2017 and February 2024, pediatric and adult patients presenting with clinical features suggestive of immunodeficiency were subjected to FCM evaluation, including lymphocyte subset analysis, detection of specific surface or intracellular proteins, and functional analysis of immune cells. Results: Over a nearly seven-year period, our laboratory diagnosed a total of 670 patients (372 (55.5%) males and 298 (44.5%) females), distributed into 70 different IEIs belonging to 9 different categories of the International Union of Immunological Societies classification. FCM was used to diagnose and categorize IEI in 514 patients (76.7%). It provided direct diagnostic insights for IEIs such as severe combined immunodeficiency, Omenn syndrome, MHC class II deficiency, familial hemophagocytic lymphohistiocytosis, and CD55 deficiency. For certain IEIs, including hyper-IgE syndrome, STAT1-gain of function, autoimmune lymphoproliferative syndrome, and activated PI3K delta syndrome, FCM offered suggestive evidence, necessitating subsequent genetic testing for confirmation. Protein expression and functional assays played a crucial role in establishing definitive diagnoses for various disorders. To setup such diagnostic assays at high and reproducible quality, high level of expertise is required; in house reference values need to be determined and the parallel testing of healthy controls is highly recommended. Conclusion: Flow cytometry has emerged as a highly valuable and cost-effective tool for diagnosing and studying most IEIs, particularly in low-income countries where access to genetic testing can be limited. FCM analysis could provide direct diagnostic insights for most common IEIs, offer clues to the underlying genetic defects, and/or aid in narrowing the list of putative genes to be analyzed.

Indexed as

Flow CytometryAdolescentAdultAlgeriaChildChild, PreschoolFemaleHumansImmunologic Deficiency SyndromesImmunophenotypingInfantInfant, NewbornMaleRetrospective StudiesYoung Adultcomplement deficienciesflow cytometryflow cytometry-based diagnostic approachfunctional assayslymphocyte phenotypingprotein expression assays

Identifiers

PMID39072316
PMCPMC11273131

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