Evidence map›Paper›PMID 39071429›Full record

ArticlebioRxiv : the preprint server for biology2024

Rapid protein evolution by few-shot learning with a protein language model.

Kaiyi Jiang, Zhaoqing Yan, Matteo Di Bernardo, Samantha R Sgrizzi, Lukas Villiger, Alisan Kayabolen, Byungji Kim, Josephine K Carscadden, Masahiro Hiraizumi, Hiroshi Nishimasu and 2 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Kaiyi JiangDepartment of Medicine Division of Engineering in Medicine Brigham and Women's Hospital Harvard Medical School Boston, 02115 MA, USA.
Zhaoqing YanDepartment of Medicine Division of Engineering in Medicine Brigham and Women's Hospital Harvard Medical School Boston, 02115 MA, USA.
Matteo Di BernardoDepartment of Bioengineering Massachusetts Institute of Technology Cambridge, 02139 MA, USA.
Samantha R SgrizziDepartment of Medicine Division of Engineering in Medicine Brigham and Women's Hospital Harvard Medical School Boston, 02115 MA, USA.
Lukas VilligerDepartment of Dermatology and Allergology Kantonspital St. Gallen St. Gallen, 9000, Switzerland.
Alisan KayabolenDepartment of Medicine Division of Engineering in Medicine Brigham and Women's Hospital Harvard Medical School Boston, 02115 MA, USA.
Byungji KimKoch Institute for Integrative Cancer Research At MIT Massachusetts Institute of Technology Cambridge, 02139 MA, USA.
Josephine K CarscaddenDepartment of Medicine Division of Engineering in Medicine Brigham and Women's Hospital Harvard Medical School Boston, 02115 MA, USA.
Masahiro HiraizumiDepartment of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Hiroshi NishimasuDepartment of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Jonathan S GootenbergDepartment of Medicine Division of Engineering in Medicine Brigham and Women's Hospital Harvard Medical School Boston, 02115 MA, USA.ORCID 0000-0003-2757-2175
Omar O AbudayyehDepartment of Medicine Division of Engineering in Medicine Brigham and Women's Hospital Harvard Medical School Boston, 02115 MA, USA.ORCID 0000-0002-7979-3220

Funding

Discovery and manipulation of transcription factors to restore long term stem cell repopulation in aged bone-marrowR01AG074932 · NIA · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI Omar O Abudayyeh, Jonathan Samuel Gootenberg · 2022 to 2026
$3.3M
Programmable gene integration and cell engineering with CRISPR-directed integrasesR01EB031957 · NIBIB · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI ABUDAYYEH, OMAR O, GOOTENBERG, JONATHAN SAMUEL · 2021 to 2024
$2.4M
Developing programmable RNA writing tools with the novel RNA-guided RNA-targeting CRISPR effector Cas7-11R01GM148745 · NIGMS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI Omar O Abudayyeh, Jonathan Samuel Gootenberg · 2023 to 2026
$1.9M
Systematic discovery, characterization, and design of novel genome editing and delivery tools using a high-throughput metagenomic screening pipelineR21AI149694 · NIAID · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI ABUDAYYEH, OMAR O, GOOTENBERG, JONATHAN SAMUEL · 2020 to 2021
$414k
RNA targeting tools with novel specific RNA-guided RNA-targeting CRISPR effectorsR56HG011857 · NHGRI · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI ABUDAYYEH, OMAR O, GOOTENBERG, JONATHAN SAMUEL · 2021 to 2021
$363k
NHGRI NIH HHS R56 HG011857NIAID NIH HHS R21 AI149694NIA NIH HHS R01 AG074932NIBIB NIH HHS R01 EB031957NIGMS NIH HHS R01 GM148745
6 · The paper itself

Abstract

Directed evolution of proteins is critical for applications in basic biological research, therapeutics, diagnostics, and sustainability. However, directed evolution methods are labor intensive, cannot efficiently optimize over multiple protein properties, and are often trapped by local maxima.

Identifiers

PMID39071429
PMCPMC11275896

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.