Evidence map›Paper›PMID 39071344›Full record

ArticlebioRxiv : the preprint server for biology2024

Single-cell transcriptomics reveal differences between chorionic and basal plate cytotrophoblasts and trophoblast stem cells.

Robert Morey, Francesca Soncin, Sampada Kallol, Nirvay Sah, Zoe Manalo, Tony Bui, Jaroslav Slamecka, Virginia Chu Cheung, Don Pizzo, Daniela F Requena and 7 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

17 authors.

Robert MoreyDepartment of Pathology, School of Medicine, University of California San Diego, La Jolla, CA, 92093, USA.ORCID 0000-0001-8480-4564
Francesca SoncinDepartment of Pathology, School of Medicine, University of California San Diego, La Jolla, CA, 92093, USA.
Sampada KallolDepartment of Pathology, School of Medicine, University of California San Diego, La Jolla, CA, 92093, USA.
Nirvay SahDepartment of Pathology, School of Medicine, University of California San Diego, La Jolla, CA, 92093, USA.
Zoe ManaloDepartment of Pathology, School of Medicine, University of California San Diego, La Jolla, CA, 92093, USA.
Tony BuiDepartment of Pathology, School of Medicine, University of California San Diego, La Jolla, CA, 92093, USA.
Jaroslav SlameckaDepartment of Pathology, School of Medicine, University of California San Diego, La Jolla, CA, 92093, USA.
Virginia Chu CheungDepartment of Pathology, School of Medicine, University of California San Diego, La Jolla, CA, 92093, USA.
Don PizzoDepartment of Pathology, School of Medicine, University of California San Diego, La Jolla, CA, 92093, USA.
Daniela F RequenaDepartment of Pathology, School of Medicine, University of California San Diego, La Jolla, CA, 92093, USA.
Ching-Wen ChangDepartment of Pathology, School of Medicine, University of California San Diego, La Jolla, CA, 92093, USA.
Omar FarahDepartment of Pathology, School of Medicine, University of California San Diego, La Jolla, CA, 92093, USA.
Ryan KittleDepartment of Pathology, School of Medicine, University of California San Diego, La Jolla, CA, 92093, USA.
Morgan MeadsDepartment of Pathology, School of Medicine, University of California San Diego, La Jolla, CA, 92093, USA.
Mariko HoriiDepartment of Pathology, School of Medicine, University of California San Diego, La Jolla, CA, 92093, USA.
Kathleen FischCenter for Perinatal Discovery, University of California San Diego, La Jolla, CA, 92093, USA.
Mana M ParastDepartment of Pathology, School of Medicine, University of California San Diego, La Jolla, CA, 92093, USA.

Funding

UC San Diego Clinical and Translational Research InstituteUL1TR001442 · NCATS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI FIRESTEIN, GARY S, HOGARTH, MICHAEL · 2015 to 2024
$88.3M
TRAINING IN REPRODUCTIVE SCIENCEST32HD007203 · NICHD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI PAMELA L MELLON · 1988 to 2026
$4.8M
Cellular Atlas of the Human Placenta: Structure-Function Relationships and their Implications for Placental DysfunctionR01HD104805 · NICHD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI PARAST, MANA M · 2021 to 2025
$2.8M
Trophoblast progenitor heterogeneity and function in normal and Trisomy 21-affected placentaeR01HD111787 · NICHD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Mana M Parast · 2023 to 2026
$2.6M
Role of VGLL1 in human placental development and trophoblast specificationR01HD096260 · NICHD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI SONCIN, FRANCESCA · 2019 to 2023
$2.5M
Human Trophoblast Stem Cells: the In Vivo Niche and Relationship to Pluripotent Stem CellsR01HD089537 · NICHD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI PARAST, MANA M · 2017 to 2021
$2.4M
Modeling Preeclampsia Using Human Pluripotent Stem CellsR00HD091452 · NICHD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI HORII, MARIKO · 2020 to 2022
$737k
Illumina NovaSeq 6000 Sequencing SystemS10OD026929 · OD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI JEPSEN, KRISTEN LYNN · 2019 to 2019
$600k
NCATS NIH HHS UL1 TR001442NICHD NIH HHS R00 HD091452NICHD NIH HHS R01 HD089537NICHD NIH HHS R01 HD096260NICHD NIH HHS R01 HD104805NICHD NIH HHS R01 HD111787NICHD NIH HHS T32 HD007203NIH HHS S10 OD026929
6 · The paper itself

Abstract

Cytotrophoblast (CTB) of the early gestation human placenta are bipotent progenitor epithelial cells, which can differentiate into invasive extravillous trophoblast (EVT) and multinucleated syncytiotrophoblast (STB). Trophoblast stem cells (TSC), derived from early first trimester placentae, have also been shown to be bipotential. In this study, we set out to probe the transcriptional diversity of first trimester CTB and compare TSC to various subgroups of CTB. We performed single-cell RNA sequencing on six normal placentae, four from early (6-8 weeks) and two from late (12-14 weeks) first trimester, of which two of the early first trimester cases were separated into basal (maternal) and chorionic (fetal) fractions prior to sequencing. We also sequenced three TSC lines, derived from 6-8 week placentae, to evaluate similarities and differences between primary CTB and TSC. CTB clusters displayed notable distinctions based on gestational age, with early first trimester placentae showing enrichment for specific CTB subtypes, further influenced by origin from the basal or chorionic plate. Differential expression analysis of CTB from basal versus chorionic plate highlighted pathways associated with proliferation, unfolded protein response, and oxidative phosphorylation. We identified trophoblast states representing initial progenitor CTB, precursor STB, precursor and mature EVT, and multiple CTB subtypes. CTB progenitors were enriched in early first trimester placentae, with basal plate cells biased toward EVT, and chorionic plate cells toward STB, precursors. Clustering and trajectory inference analysis indicated that TSC were most like EVT precursor cells, with only a small percentage of TSC on the pre-STB differentiation trajectory. This was confirmed by flow cytometric analysis of 6 different TSC lines, which showed uniform expression of proximal column markers ITGA2 and ITGA5. Additionally, we found that ITGA5

Identifiers

PMID39071344
PMCPMC11275976

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.