Evidence map›Paper›PMID 39071103›Full record

ArticleSmall science2023

Protein Engineering and High-Throughput Screening by Yeast Surface Display: Survey of Current Methods.

Joanan Lopez-Morales, Rosario Vanella, Elizabeth A Appelt, Sarah Whillock, Alexandra M Paulk, Eric V Shusta, Benjamin J Hackel, Chang C Liu, Michael A Nash

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Small science, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Article
  2. Yeast as a Model for Human Disease.International journal of molecular sciences · 2026
    Review
  3. Article
  4. Article
  5. Protein engineering: status report.Protein engineering, design & selection : PEDS · 2026
    Review
  6. Article
  7. Yeast-mediated display: probingIranian journal of microbiology · 2025
    Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. High-throughput protein binder discovery by rapid in vivo selection.bioRxiv : the preprint server for biology · 2025
    Article
  14. Optimized single-cell gates for yeast display screening.Protein engineering, design & selection : PEDS · 2025
    Article
  15. Review
  16. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Joanan Lopez-MoralesInstitute for Physical Chemistry, Department of Chemistry, University of Basel, Basel 4058, Switzerland; Swiss Nanoscience Institute, University of Basel, Basel 4056, Switzerland; Department of Biosystems Science and Engineering, ETH Zurich, Basel 4058, Switzerland.
Rosario VanellaInstitute for Physical Chemistry, Department of Chemistry, University of Basel, Basel 4058, Switzerland; Department of Biosystems Science and Engineering, ETH Zurich, Basel 4058, Switzerland.
Elizabeth A AppeltDepartment of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI 53706, USA.
Sarah WhillockDepartment of Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455, USA.
Alexandra M PaulkProgram in Mathematical, Computational, and Systems Biology, University of California, Irvine, CA 92697-2280, USA; Center for Synthetic Biology, University of California, Irvine, CA 92697, USA; Department of Biomedical Engineering, University of California, Irvine, CA 92697, USA.
Eric V ShustaDepartment of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI 53706, USA; Department of Neurological Surgery, University of Wisconsin-Madison, Madison, WI 53706, USA.
Benjamin J HackelDepartment of Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455, USA; Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, MN 55455, USA.
Chang C LiuDepartment of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA; Department of Chemistry, University of California, Irvine, CA 92697, USA; Center for Synthetic Biology, University of California, Irvine, CA 92697, USA; Department of Biomedical Engineering, University of California, Irvine, CA 92697, USA.
Michael A NashInstitute for Physical Chemistry, Department of Chemistry, University of Basel, Basel 4058, Switzerland; Swiss Nanoscience Institute, University of Basel, Basel 4056, Switzerland; Department of Biosystems Science and Engineering, ETH Zurich, Basel 4058, Switzerland.

Funding

Biotechnology Training ProgramT32GM135066 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI SCOTT M. COYLE, BRIAN G FOX · 2020 to 2026
$7.0M
Mentor Training to enhance mentorship in an interdisciplinary training programT32GM136624 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI Arthur D Lander, Qing Nie · 2020 to 2026
$3.1M
New Human Antibodies for CNS Drug DeliveryR01NS118028 · NINDS · UNIVERSITY OF WISCONSIN-MADISON · PI SHUSTA, ERIC V · 2021 to 2025
$1.9M
NIGMS NIH HHS T32 GM135066NIGMS NIH HHS T32 GM136624NINDS NIH HHS R01 NS118028
6 · The paper itself

Abstract

Yeast surface display (YSD) is a powerful tool in biotechnology that links genotype to phenotype. In this review, the latest advancements in protein engineering and high-throughput screening based on YSD are covered. The focus is on innovative methods for overcoming challenges in YSD in the context of biotherapeutic drug discovery and diagnostics. Topics ranging from titrating avidity in YSD using transcriptional control to the development of serological diagnostic assays relying on serum biopanning and mitigation of unspecific binding are covered. Screening techniques against nontraditional cellular antigens, such as cell lysates, membrane proteins, and extracellular matrices are summarized and techniques are further delved into for expansion of the chemical repertoire, considering protein-small molecule hybrids and noncanonical amino acid incorporation. Additionally, in vivo gene diversification and continuous evolution in yeast is discussed. Collectively, these techniques enhance the diversity and functionality of engineered proteins isolated via YSD, broadening the scope of applications that can be addressed. The review concludes with future perspectives and potential impact of these advancements on protein engineering. The goal is to provide a focused summary of recent progress in the field.

Indexed as

biotechnologydiagnosticshigh-throughput screeningpharmaceuticalsprotein engineeringSaccharomyces cerevisiaeyeast surface display

Identifiers

PMID39071103
PMCPMC11271970

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.