Evidence map›Paper›PMID 39070629›Full record

ArticleResearch square2024

Characterization and structural basis for the brightness of mCLIFY: a novel monomeric and circularly permuted bright yellow fluorescent protein.

Him Shweta, Kushol Gupta, Yufeng Zhou, Xiaonan Cui, Selene Li, Zhe Lu, Yale E Goldman, Jody A Dantzig

Abstract readPreprint
In one paragraph

Article in Research square, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Him ShwetaPennsylvania Muscle Institute, University of Pennsylvania, Philadelphia, PA-19104, United States of America.
Kushol GuptaDepartment of Biochemistry & Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA-19104, United States of America.
Yufeng ZhouDepartment of Physiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA-19104, United States of America.
Xiaonan CuiDepartment of Physiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA-19104, United States of America.
Selene LiDepartment of Physiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA-19104, United States of America.
Zhe LuDepartment of Physiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA-19104, United States of America.
Yale E GoldmanPennsylvania Muscle Institute, University of Pennsylvania, Philadelphia, PA-19104, United States of America.
Jody A DantzigPennsylvania Muscle Institute, University of Pennsylvania, Philadelphia, PA-19104, United States of America.

Funding

X-ray Scattering Technology CoreP30GM133893 · NIGMS · BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB · PI Sean McSweeney · 2019 to 2026
$38.6M
Permeation of Inward Rectifier K+ ChannelsR01GM055560 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI LU, ZHE · 1997 to 2024
$8.4M
Supplemental Application linked to parent grant Structural Dynamics of Molecular Motors, the Cytoskeleton, and the RibosomeR35GM118139 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI YALE E GOLDMAN · 2016 to 2026
$8.3M
Kinetic mechanisms of amino acid transportersR01DK125521 · NIDDK · UNIVERSITY OF PENNSYLVANIA · PI LU, ZHE · 2020 to 2023
$1.6M
A Pixel Array Detector System for Small Angle X-ray ScatteringS10OD018483 · OD · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI CLASSEN, SCOTT · 2014 to 2014
$998k
Multi-Parameter Fluorescence Detection Single Molecule MicroscopyS10OD021573 · OD · UNIVERSITY OF PENNSYLVANIA · PI GOLDMAN, YALE E · 2017 to 2017
$484k
NIDDK NIH HHS R01 DK125521NIGMS NIH HHS P30 GM133893NIGMS NIH HHS R01 GM055560NIGMS NIH HHS R35 GM118139NIH HHS S10 OD018483NIH HHS S10 OD021573
6 · The paper itself

Abstract

We present mCLIFY: a monomeric, bright, yellow, and long-lived fluorescent protein (FP) created by circular permutation of YPet, the brightest yellow FP from

Indexed as

analytical ultracentrifugationcircular permutationfluorescence lifetimefluorescence resonance energy transferFluorescent proteinssmall-angle X-ray scatteringX-ray crystallography

Identifiers

PMID39070629
PMCPMC11276004

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.