Evidence map›Paper›PMID 39070090›Full record

ArticleJournal of chemical education2024

From Molecules to Classrooms: A Comprehensive Guide to Single-Molecule Localization Microscopy.

Guangjie Yan, Yuteng Zhang, Alexis Allamprese, Kameron N Brooks, Wenkai Chen, Shudan Yan, Tai-Yen Chen

Abstract read
In one paragraph

Article in Journal of chemical education, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Guangjie YanDepartment of Chemistry, University of Houston, Houston, Texas 77204.
Yuteng ZhangDepartment of Chemistry, University of Houston, Houston, Texas 77204.
Alexis AllampreseDepartment of Chemistry, University of Houston, Houston, Texas 77204.
Kameron N BrooksDepartment of Chemistry, University of Houston, Houston, Texas 77204.
Wenkai ChenDepartment of Chemistry, University of Houston, Houston, Texas 77204.
Shudan YanDepartment of Chemistry, University of Houston, Houston, Texas 77204.
Tai-Yen ChenDepartment of Chemistry, University of Houston, Houston, Texas 77204.

Funding

Quantitative Cu-Homeostasis in Live Mammalian Cells at the Single-Molecule LevelR35GM133505 · NIGMS · UNIVERSITY OF HOUSTON · PI CHEN, TAI-YEN · 2019 to 2023
$2.1M
NIGMS NIH HHS R35 GM133505
6 · The paper itself

Abstract

Single-molecule localization microscopy (SMLM) has revolutionized our ability to visualize cellular structures, offering unprecedented detail. However, the intricate biophysical principles that underlie SMLM can be daunting for newcomers, particularly undergraduate and graduate students. To address this challenge, we introduce the fundamental concepts of SMLM, providing a solid theoretical foundation. In addition, we have developed an intuitive graphical interface APP that simplifies these core concepts, making them more accessible for students. This APP clarifies how super-resolved images are fitted and highlights the crucial factors determining image quality. Our approach deepens students' understanding of SMLM by combining theoretical instruction with practical learning. This development equips them with the skills to carry out single-molecule super-resolved experiments and explore the microscopic world beyond the diffraction limit.

Indexed as

Image formationImage QuantificationPoint-Spread FunctionSpatial ResolutionWave Optics

Identifiers

PMID39070090
PMCPMC11271931

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.