Evidence map›Paper›PMID 39069984›Full record

ArticleACS physical chemistry Au2024

Rational Design of Far-Red Archaerhodopsin-3-Based Fluorescent Genetically Encoded Voltage Indicators: from Elucidation of the Fluorescence Mechanism in Archers to Novel Red-Shifted Variants.

Dmitrii M Nikolaev, Vladimir N Mironov, Ekaterina M Metelkina, Andrey A Shtyrov, Andrey S Mereshchenko, Nikita A Demidov, Sergey Yu Vyazmin, Tatiana B Tennikova, Svetlana E Moskalenko, Stanislav A Bondarev and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS physical chemistry Au, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Fluorescence of the Retinal Chromophore in Microbial and Animal Rhodopsins.International journal of molecular sciences · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Dmitrii M NikolaevInstitute of Chemistry, Saint Petersburg State University, 26 Universitetskii pr, St. Petersburg 198504, Russia.
Vladimir N MironovSaint Petersburg Academic University, 8/3 Khlopina Street, St. Petersburg 194021, Russia.
Ekaterina M MetelkinaInstitute of Chemistry, Saint Petersburg State University, 26 Universitetskii pr, St. Petersburg 198504, Russia.
Andrey A ShtyrovInstitute of Chemistry, Saint Petersburg State University, 26 Universitetskii pr, St. Petersburg 198504, Russia.
Andrey S MereshchenkoInstitute of Chemistry, Saint Petersburg State University, 26 Universitetskii pr, St. Petersburg 198504, Russia.ORCID https://orcid.org/0000-0001-9390-1446
Nikita A DemidovSaint Petersburg Academic University, 8/3 Khlopina Street, St. Petersburg 194021, Russia.
Sergey Yu VyazminSaint Petersburg Academic University, 8/3 Khlopina Street, St. Petersburg 194021, Russia.
Tatiana B TennikovaInstitute of Chemistry, Saint Petersburg State University, 26 Universitetskii pr, St. Petersburg 198504, Russia.
Svetlana E MoskalenkoDepartment of Genetics and Biotechnology, Saint Petersburg State University, 7/9 Universitetskaya emb, St. Petersburg 199034, Russia.
Stanislav A BondarevDepartment of Genetics and Biotechnology, Saint Petersburg State University, 7/9 Universitetskaya emb, St. Petersburg 199034, Russia.ORCID https://orcid.org/0000-0003-3137-944X
Galina A ZhouravlevaDepartment of Genetics and Biotechnology, Saint Petersburg State University, 7/9 Universitetskaya emb, St. Petersburg 199034, Russia.
Andrey V VasinInstitute of Biomedical Systems and Biotechnologies, Peter the Great St. Petersburg Polytechnic University, 29 Polytechnicheskaya Str., St. Petersburg 195251, Russia.
Maxim S PanovInstitute of Chemistry, Saint Petersburg State University, 26 Universitetskii pr, St. Petersburg 198504, Russia.
Mikhail N RyazantsevInstitute of Chemistry, Saint Petersburg State University, 26 Universitetskii pr, St. Petersburg 198504, Russia.ORCID https://orcid.org/0000-0003-3413-1706

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Genetically encoded voltage indicators (GEVIs) have found wide applications as molecular tools for visualization of changes in cell membrane potential. Among others, several classes of archaerhodopsin-3-based GEVIs have been developed and have proved themselves promising in various molecular imaging studies. To expand the application range for this type of GEVIs, new variants with absorption band maxima shifted toward the first biological window and enhanced fluorescence signal are required. Here, we integrate computational and experimental strategies to reveal structural factors that distinguish far-red bright archaerhodopsin-3-based GEVIs, Archers, obtained by directed evolution in a previous study (McIsaac et al.,

Identifiers

PMID39069984
PMCPMC11274289

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.