Evidence map›Paper›PMID 39069977›Full record

ArticleACS physical chemistry Au2024

Measuring Vesicle Loading with Holographic Microscopy and Bulk Light Scattering.

Lan Hai Anh Tran, Lauren A Lowe, Yaam Deckel, Matthew Turner, James Luong, Omar Abdullah A Khamis, Megan L Amos, Anna Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS physical chemistry Au, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Lan Hai Anh TranSchool of Chemistry, UNSW, Sydney 2052, NSW, Australia.ORCID https://orcid.org/0000-0003-3646-562X
Lauren A LoweSchool of Chemistry, UNSW, Sydney 2052, NSW, Australia.
Yaam DeckelSchool of Chemistry, UNSW, Sydney 2052, NSW, Australia.
Matthew TurnerSchool of Chemistry, UNSW, Sydney 2052, NSW, Australia.ORCID https://orcid.org/0009-0006-6751-9773
James LuongSchool of Chemistry, UNSW, Sydney 2052, NSW, Australia.
Omar Abdullah A KhamisSchool of Chemistry, UNSW, Sydney 2052, NSW, Australia.
Megan L AmosSchool of Chemistry, UNSW, Sydney 2052, NSW, Australia.
Anna WangSchool of Chemistry, UNSW, Sydney 2052, NSW, Australia.ORCID https://orcid.org/0000-0002-2148-1996

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We report efforts to quantify the loading of cell-sized lipid vesicles using in-line digital holographic microscopy. This method does not require fluorescent reporters, fluorescent tracers, or radioactive tracers. A single-color LED light source takes the place of conventional illumination to generate holograms rather than bright field images. By modeling the vesicle's scattering in a microscope with a Lorenz-Mie light scattering model and comparing the results to data holograms, we are able to measure the vesicle's refractive index and thus loading. Performing the same comparison for bulk light scattering measurements enables the retrieval of vesicle loading for nanoscale vesicles.

Identifiers

PMID39069977
PMCPMC11274288

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.