Evidence map›Paper›PMID 39068631›Full record

ArticleThe protein journal2024

Mechanistic Insights into How the Single Point Mutation Change the Autoantibody Repertoire.

Zhong Ni, Fangyuan Song, Huimin Zhou, Ying Xu, Zhiguo Wang, Dongfeng Chen

Abstract read
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In one paragraph

Article in The protein journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Zhong NiSchool of Life Sciences, Jiangsu University, Zhenjiang, 212013, China.
Fangyuan SongSchool of Life Sciences, Jiangsu University, Zhenjiang, 212013, China.
Huimin ZhouSchool of Life Sciences, Jiangsu University, Zhenjiang, 212013, China.
Ying XuSchool of Life Sciences, Jiangsu University, Zhenjiang, 212013, China.
Zhiguo WangInstitute of Ageing Research, School of Medicine, Hangzhou Normal University, Hangzhou, Zhejiang, 311121, China.
Dongfeng ChenSchool of Life Sciences, Jiangsu University, Zhenjiang, 212013, China. dongfeng@ujs.edu.cn.ORCID https://orcid.org/0000-0003-1910-1234

Funding

Jiangsu University 18JDG028
6 · The paper itself

Abstract

A recent study showed that just one point mutation F33 to Y in the complementarity-determining region 1 of heavy chain (H-CDR1) could lead to the auto-antibody losing its DNA binding ability. However, the potential molecular mechanisms have not been well elucidated. In this study, we investigated how the antibody lost the DNA binding ability caused by mutation F33 to Y in the H-CDR1. We found that the electrostatic force was not the primary driving force for the interaction between anti-DNA antibodies and the antigen single strand DNA (ssDNA), and that the H-CDR2 largely contributed to the binding of antigen ssDNA, even larger than H-CDR1. The H-F33Y mutation could increase the hydrogen-bond interaction but impair the pi-pi stacking interaction between the antibody and ssDNA. We further found that F33

Indexed as

Complementarity Determining RegionsDNA, Single-StrandedPoint MutationAnimalsAutoantibodiesCHO CellsCricetulusHumansImmunoglobulin Heavy ChainsAutoantibodiesComplementarity Determining RegionsDNA, Single-StrandedImmunoglobulin Heavy ChainsAutoantibodyMolecular dynamic simulationMutationPi-pi stackingRepertoire

Identifiers

PMID39068631

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.