Evidence map›Paper›PMID 39068357›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Efficient and Powerful Integration of Targeted Metabolomics and Transcriptomics for Analyzing the Metabolism Behind Desirable Traits in Plants.

Christopher R Johnston, Cintia L Arias, Ana Paula Alonso

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Christopher R JohnstonDepartment of Biological Sciences & BioDiscovery Institute, University of North Texas, Denton, TX, USA.
Cintia L AriasDepartment of Biological Sciences & BioDiscovery Institute, University of North Texas, Denton, TX, USA.
Ana Paula AlonsoDepartment of Biological Sciences & BioDiscovery Institute, University of North Texas, Denton, TX, USA. Anapaula.Alonso@unt.edu.

Funding

Agricultural and Food Research Initiative (AFRI) 2021-67034-35137Department of Energy # DE-SC0020325USDA National Institute of Food and Agriculture 2021-67013-3777
6 · The paper itself

Abstract

Through current mass spectrometry methods and multiple RNA-Seq technologies, large metabolomics and transcriptomics datasets are readily obtainable, which provide a powerful and global perspective on metabolism. Indeed, one "omics" method is often not enough to draw strong conclusions about metabolism. Combining and interpreting multiple "omics" datasets remains a challenging task that requires careful statistical considerations and pre-planning. Here we describe a protocol for obtaining high-quality metabolomics and transcriptomics datasets in developing plant embryos followed by a robust approach to integration of the two. This protocol is readily adjustable and scalable to any other metabolically active organ or tissue.

Indexed as

MetabolomicsPlantsTranscriptomeGene Expression ProfilingGene Expression Regulation, PlantMass SpectrometryMetabolomeDifferential expressionLC-MS/MSMetabolomicsMulti-omicsOverrepresentation analysisPlant metabolismRNA-SeqTranscriptomics

Identifiers

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.