Evidence map›Paper›PMID 39068342›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

TED: Enhancing Translation Efficiency in Bacterial Expression Systems.

Tomo Kondo, Takayuki Shimizu

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Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Tomo KondoDepartment of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo, Japan. tomokondo@g.ecc.u-tokyo.ac.jp.
Takayuki ShimizuResearch Group of Biological Sciences, Division of Natural Sciences, Nara Women's University, Kitauoya-nishimachi, Nara, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Genetic engineering enables the forced expression of desired products in bacteria, which can then be used for a variety of applications, including functional analysis and pharmaceuticals. Here, we describe a method for tuning translation in bacteria, including Escherichia coli and Rhodobacter capsulatus, based on a phenomenon known as TED (translation enhancement by a Dictyostelium gene sequence). This method promotes translation of mRNA encoded by downstream genes by inserting a short nucleotide sequence into the 5' untranslated region between the promoter and the Shine-Dalgarno (SD) sequence. Various expression levels can be observed depending on the inserted sequence and its length, even with an identical promoter.

Indexed as

Escherichia coliProtein Biosynthesis5' Untranslated RegionsDictyosteliumGene Expression Regulation, BacterialGenetic EngineeringPromoter Regions, GeneticRhodobacter capsulatusRNA, Messenger5' Untranslated RegionsRNA, MessengerDictyosteliumEscherichia coliHeterologous expressionRhodobacter capsulatusTranslation

Identifiers

PMID39068342

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.