Evidence map›Paper›PMID 39066900›Full record

ArticleMikrochimica acta2024

Modulable 3D-printed plantibody-laden platform enabling microscale affinity extraction and ratiometric front-face fluorescence detection of microcystin-LR in marine waters.

Roser Payà-Pou, Julia Aguirre-Camacho, Ernesto Francisco Simó-Alfonso, Dietmar Knopp, Manuel Miró, Enrique Javier Carrasco-Correa

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Mikrochimica acta, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Roser Payà-PouCLECEM Group, Department of Analytical Chemistry, University of Valencia, C/ Doctor Moliner, 50, 46100, Burjassot, Valencia, Spain.
Julia Aguirre-CamachoCLECEM Group, Department of Analytical Chemistry, University of Valencia, C/ Doctor Moliner, 50, 46100, Burjassot, Valencia, Spain.
Ernesto Francisco Simó-AlfonsoCLECEM Group, Department of Analytical Chemistry, University of Valencia, C/ Doctor Moliner, 50, 46100, Burjassot, Valencia, Spain.
Dietmar KnoppDepartment of Chemistry, Chair of Analytical Chemistry and Water Chemistry, Technical University Munich, TUM School of Natural Sciences, Lichtenbergstrasse 4, 85748, Garching, Germany.
Manuel MiróFI-TRACE Group, Department of Chemistry, University of the Balearic Islands, Carretera de Valldemossa, km 7.5, Palma, 07122, Spain. manuel.miro@uib.es.ORCID http://orcid.org/0000-0002-8413-3008
Enrique Javier Carrasco-CorreaCLECEM Group, Department of Analytical Chemistry, University of Valencia, C/ Doctor Moliner, 50, 46100, Burjassot, Valencia, Spain. enrique.carrasco@uv.es.

Funding

Conselleria de Cultura, Educación y Ciencia, Generalitat Valenciana CIAICO/2022/183Conselleria de Cultura, Educación y Ciencia, Generalitat Valenciana CPI-20-446Conselleria de Cultura, Educación y Ciencia, Generalitat Valenciana INVEST/2022/425Conselleria de Cultura, Educación y Ciencia, Generalitat Valenciana MFA/2022/034Ministerio de Ciencia, Innovación y Universidades PID2020-117686RB-C33Ministerio de Ciencia, Innovación y Universidades PID2021-125459OB-I00Ministerio de Ciencia, Innovación y Universidades TED2021-131303B-I00
6 · The paper itself

Abstract

A 3D-printed stereolithographic platform for selective biorecognition is designed to enable convective microscale affinity extraction of microcystin-LR (MC-LR) followed by direct solid-phase optosensing exploiting ratiometric front-face fluorescence spectroscopy. For this purpose, a recombinant monoclonal plantibody (recAb) is covalently attached to a 3D-printed structure for sorptive immunoextraction, whereupon the free and unbound primary amino moieties of the recAb are derivatized with a fluorescent probe. The fluorophore-recAb-MC-LR laden device is then accommodated in the cuvette holder of a conventional fluorometer without any instrumental modification for the recording of the solid-phase fluorescence emission. Using Rodbard's four-parameter sigmoidal function, the 3D-printed bioselective platform features a limit of detection (LOD) of 28 ng L

Indexed as

Marine ToxinsMicrocystinsPrinting, Three-DimensionalSeawaterSpectrometry, FluorescenceAntibodies, MonoclonalFluorescent DyesLimit of DetectionRecombinant ProteinsAntibodies, Monoclonalcyanoginosin LRFluorescent DyesMarine ToxinsMicrocystinsRecombinant Proteins3D printingEmerging contaminantsImmunoassaySeawater

Identifiers

PMID39066900
PMCPMC11283425

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.