Evidence map›Paper›PMID 39066179›Full record

ArticleViruses2024

Development of Dry and Liquid Duplex Reagent Mix-Based Polymerase Chain Reaction Assays as Novel Tools for the Rapid and Easy Quantification of Bovine Leukemia Virus (BLV) Proviral Loads.

Sonoko Watanuki, Kazuyuki Shoji, Masaki Izawa, Mitsuaki Okami, Yingbao Ye, Aronggaowa Bao, Yulin Liu, Etsuko Saitou, Kimikazu Sugiyama, Michiru Endo and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. First Identification ofPathogens (Basel, Switzerland) · 2025
    Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Sonoko WatanukiLaboratory of Global Infectious Diseases Control Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.ORCID 0009-0006-8408-2622
Kazuyuki ShojiMolecular Diagnosis Division, Nippon Gene Co., Ltd., 2-8-16 Toiya-machi, Toyama 930-0834, Japan.
Masaki IzawaMolecular Diagnosis Division, Nippon Gene Co., Ltd., 2-8-16 Toiya-machi, Toyama 930-0834, Japan.
Mitsuaki OkamiMolecular Diagnosis Division, Nippon Gene Co., Ltd., 2-8-16 Toiya-machi, Toyama 930-0834, Japan.
Yingbao YeLaboratory of Global Infectious Diseases Control Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.ORCID 0009-0002-8799-7327
Aronggaowa BaoLaboratory of Global Infectious Diseases Control Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.ORCID 0009-0001-2424-4937
Yulin LiuLaboratory of Global Infectious Diseases Control Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.ORCID 0009-0003-5811-9418
Etsuko SaitouHyogo Prefectural Awaji Meat Inspection Center, 49-18 Shitoorinagata, Minamiawaji 656-0152, Japan.
Kimikazu SugiyamaKumagaya Livestock Hygiene Service Center, Kumagaya 360-0813, Japan.
Michiru EndoKumagaya Livestock Hygiene Service Center, Kumagaya 360-0813, Japan.
Yasunobu MatsumotoLaboratory of Global Infectious Diseases Control Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Yoko AidaLaboratory of Global Infectious Diseases Control Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.ORCID 0000-0001-7400-3587

Funding

Grant-in-Aid for JSPS Fellow from the Japan Society for the Promotion of Science (JSPS) 23KJ0412Japan Racing Association (JRA) grant from the Livestock Promotional SubsidyNippon Gene Co., LTD. a collaborative research fund
6 · The paper itself

Abstract

Bovine leukemia virus (BLV) is prevalent worldwide, causing serious problems in the cattle industry. The BLV proviral load (PVL) is a useful index for estimating disease progression and transmission risk. We previously developed a quantitative real-time PCR (qPCR) assay to measure the PVL using the coordination of common motif (CoCoMo) degenerate primers. Here, we constructed a novel duplex BLV-CoCoMo qPCR assay that can amplify two genes simultaneously using a FAM-labeled MGB probe for the BLV LTR gene and a VIC-labeled MGB probe for the

Indexed as

Enzootic Bovine LeukosisLeukemia Virus, BovineProvirusesReal-Time Polymerase Chain ReactionSensitivity and SpecificityViral LoadAnimalsCattleDNA PrimersMultiplex Polymerase Chain ReactionReproducibility of ResultsDNA Primersbovine leukemia virus (BLV)dryduplexliquidproviral load (PVL)quantitative real-time PCR (qPCR)singleplex

Identifiers

PMID39066179
PMCPMC11281531

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.