Preparation and Epitope Identification of Monoclonal Antibodies against the NS6 Protein of Porcine Deltacoronavirus (PDCoV).

Huiguang Wu, Xian Sun, Chen Li, Sihan Xie, Zhenhai Chen

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Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

5 authors.

Huiguang WuCollege of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.ORCID 0000-0001-7724-7835

Xian SunCollege of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.

Chen LiCollege of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.

Sihan XieCollege of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.

Zhenhai ChenCollege of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.

Funding

111 Project D18007Agricultural Science and Technology Independent Innovation Fund of Jiangsu Province CX (21) 2014Guangxi Key Research and Development Plan AB21238003Natural Science Foundation Projects of Jiangsu Province BK20211120Priority Academic Program Development of Jiangsu Higher Education Institutions PAPDYangzhou City and Yangzhou University corporation YZ2022187

6 · The paper itself

Abstract

Porcine deltacoronavirus (PDCoV) is an emerging enteric pathogen that causes substantial economic losses in the swine industry worldwide. The PDCoV NS6 protein is an accessory protein that plays a pivotal role in the viral life cycle and immune evasion. However, the functions of NS6 and its role in PDCoV pathogenesis remain largely unknown. In this study, we prepared a monoclonal antibody (mAb) 5-A11 that specifically recognizes the PDCoV NS6 protein. The mAb 5-A11 exhibited high specificity for PDCoV, with no cross-reactivity with several major porcine pathogenic viruses. Furthermore, the epitope recognized by mAb 5-A11 was precisely mapped to residues

Indexed as

Antibodies, MonoclonalDeltacoronavirusEpitopesViral Nonstructural ProteinsAmino Acid SequenceAnimalsAntibodies, ViralCoronavirus InfectionsEpitope MappingMiceMice, Inbred BALB CSwineSwine DiseasesAntibodies, MonoclonalAntibodies, ViralEpitopesViral Nonstructural Proteinsantigenic epitopemonoclonal antibodyNS6 proteinporcine deltacoronavirus

Identifiers

PMID39062886

DOI10.3390/ijms25147645

PMCPMC11276995

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