Evidence map›Paper›PMID 39062851›Full record

ArticleInternational journal of molecular sciences2024

Location Is Everything: Influence of His-Tag Fusion Site on Properties of Adenylosuccinate Synthetase from

Marija Zora Mišković, Marta Wojtyś, Maria Winiewska-Szajewska, Beata Wielgus-Kutrowska, Marija Matković, Darija Domazet Jurašin, Zoran Štefanić, Agnieszka Bzowska, Ivana Leščić Ašler

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Marija Zora MiškovićDepartment of Chemistry, Faculty of Science, University of Zagreb, Horvatovac 102a, HR-10000 Zagreb, Croatia.
Marta WojtyśDivision of Biophysics, Institute of Experimental Physics, Faculty of Physics, University of Warsaw, Pasteura 5, 02-093 Warsaw, Poland.
Maria Winiewska-SzajewskaDivision of Biophysics, Institute of Experimental Physics, Faculty of Physics, University of Warsaw, Pasteura 5, 02-093 Warsaw, Poland.ORCID 0000-0003-0986-6882
Beata Wielgus-KutrowskaDivision of Biophysics, Institute of Experimental Physics, Faculty of Physics, University of Warsaw, Pasteura 5, 02-093 Warsaw, Poland.ORCID 0000-0002-5049-864X
Marija MatkovićDivision of Organic Chemistry and Biochemistry, Ruđer Bošković Institute, Bijenička cesta 54, HR-10000 Zagreb, Croatia.
Darija Domazet JurašinDivision of Physical Chemistry, Ruđer Bošković Institute, Bijenička cesta 54, HR-10000 Zagreb, Croatia.ORCID 0000-0001-5261-5961
Zoran ŠtefanićDivision of Physical Chemistry, Ruđer Bošković Institute, Bijenička cesta 54, HR-10000 Zagreb, Croatia.ORCID 0000-0002-3486-4291
Agnieszka BzowskaDivision of Biophysics, Institute of Experimental Physics, Faculty of Physics, University of Warsaw, Pasteura 5, 02-093 Warsaw, Poland.ORCID 0000-0002-3976-429X
Ivana Leščić AšlerDivision of Physical Chemistry, Ruđer Bošković Institute, Bijenička cesta 54, HR-10000 Zagreb, Croatia.ORCID 0000-0002-0826-7493

Funding

Croatian Science Foundation IP-2013-11-7423Croatian Science Foundation IP-2019-04-6764ERDF POIG.02.01.00-14-122/09ERDF POIG.02.02.00-00-025/09National Science Center Harmonia 2015/18/M/NZ1/00776Polish Ministry for Science and Higher Education 501-D111-01-1110102University of Warsaw IDUB PSP-501-D111-20-0004316
6 · The paper itself

Abstract

The requirement for fast and dependable protein purification methods is constant, either for functional studies of natural proteins or for the production of biotechnological protein products. The original procedure has to be formulated for each individual protein, and this demanding task was significantly simplified by the introduction of affinity tags.

Indexed as

Adenylosuccinate SynthaseHelicobacter pyloriHistidineBacterial ProteinsCircular DichroismKineticsRecombinant Fusion ProteinsX-Ray DiffractionAdenylosuccinate SynthaseBacterial ProteinsHistidineRecombinant Fusion Proteinsadenylosuccinate synthetaseenzyme kineticsHelicobacter pyloriHis-tagprotein stabilityprotein structure

Identifiers

PMID39062851
PMCPMC11276676

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.