Study on a Mechanism of Improving MaAPX1 Protein Activity by Mutating Methionine to Lysine.

Lu Xiao, Guoxiang Jiang, Hongmei Lai, Xiaoyan Duan, Huiling Yan, Shaoge Chen, Zexin Chen, Xuewu Duan

Abstract read

In one paragraph

Article in Antioxidants (Basel, Switzerland), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

8 authors.

Lu XiaoGuangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.

Guoxiang JiangGuangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.ORCID 0000-0003-0487-8067

Hongmei LaiGuangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.

Xiaoyan DuanGuangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.

Huiling YanSchool of Food and Biological Engineering, Chengdu University, Chengdu 610106, China.

Shaoge ChenGuangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.

Zexin ChenAccurate International Biotechnology Co., Ltd., Guangzhou 510535, China.ORCID 0000-0002-9628-4567

Xuewu DuanGuangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.

Funding

National Natural Science Foundation of China 32102452Natural Science Foundation of Basic and Applied Basic Research of Guangdong Province 2024A1515012849Special Fund for Scientific Innovation Strategy-Construction of High-level Academy of Agriculture Science R2021YJ-YB-3024Youth Mentorship Program of Guangdong Academy of Agricultural Sciences R2021QD-020

6 · The paper itself

Abstract

Ascorbate peroxidases (APXs) are key components of the ascorbate-glytathione cycle, which plays an important role in removing excess reactive oxygen species (ROS) in plants. Herein, MaAPX1 was verified as being involved in the ripening and senescence of banana fruit, exhibiting responsiveness to the accumulation of ROS and the oxidation of proteins. Site-directed mutation was applied to explore the mechanism of MaAPX1 activity changes. We found that the 32-site cysteine (Cys, C) served as a potential S-nitrosylation site. The mutant MaAPX1

Indexed as

activityascorbate peroxidase 1 (APX1)enzyme kineticsmolecular dockingS-nitrosylation

Identifiers

PMID39061911

DOI10.3390/antiox13070843

PMCPMC11273533

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.