Evidence map›Paper›PMID 39061809›Full record

ArticleBioengineering (Basel, Switzerland)2024

Production of Reverse Transcriptase and DNA Polymerase in Bacterial Expression Systems.

Kristína Hriňová, Johana Dlapová, Bohuš Kubala, Ľubica Kormanová, Zdenko Levarski, Eva Struhárňanská, Ján Turňa, Stanislav Stuchlík

Abstract read
In one paragraph

Article in Bioengineering (Basel, Switzerland), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Kristína HriňováDepartment of Molecular Biology, Faculty of Natural Sciences, Comenius University in Bratislava, 84215 Bratislava, Slovakia.ORCID 0009-0003-0475-8249
Johana DlapováDepartment of Molecular Biology, Faculty of Natural Sciences, Comenius University in Bratislava, 84215 Bratislava, Slovakia.ORCID 0009-0007-1577-5684
Bohuš KubalaLaboratory for Microbial Ecology, Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská Cesta 21, 84551 Bratislava, Slovakia.
Ľubica KormanováDepartment of Molecular Biology, Faculty of Natural Sciences, Comenius University in Bratislava, 84215 Bratislava, Slovakia.
Zdenko LevarskiDepartment of Molecular Biology, Faculty of Natural Sciences, Comenius University in Bratislava, 84215 Bratislava, Slovakia.ORCID 0000-0002-9824-9140
Eva StruhárňanskáDepartment of Molecular Biology, Faculty of Natural Sciences, Comenius University in Bratislava, 84215 Bratislava, Slovakia.
Ján TurňaDepartment of Molecular Biology, Faculty of Natural Sciences, Comenius University in Bratislava, 84215 Bratislava, Slovakia.
Stanislav StuchlíkDepartment of Molecular Biology, Faculty of Natural Sciences, Comenius University in Bratislava, 84215 Bratislava, Slovakia.ORCID 0000-0001-9510-4061

Funding

Slovak Research and Development Agency APVV-17-0333Slovak Research and Development Agency APVV-21-0215Slovak Research and Development Agency APVV-21-0227Slovak Research and Development Agency APVV-22-0161
6 · The paper itself

Abstract

DNA amplification and reverse transcription enzymes have proven to be invaluable in fast and reliable diagnostics and research applications because of their processivity, specificity, and robustness. Our study focused on the production of mutant Taq DNA polymerase and mutant M-MLV reverse transcriptase in the expression hosts

Indexed as

DNA polymeraseEscherichia coliextracellular productionprotein productionreverse transcriptaseVibrio natriegens

Identifiers

PMID39061809
PMCPMC11274053

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.