Evidence map›Paper›PMID 39060946›Full record

ArticleJournal of translational medicine2024

GRASLND regulates melanoma cell progression by targeting the miR-218-5p/STAM2 axis.

Aiwei Ma, Wenqi Shi, Liyun Chen, Zijian Huang, Yiwen Zhang, Zixuan Tang, Wenshi Jiang, Mengjing Xu, Jianda Zhou, Wancong Zhang and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of translational medicine, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. mBiology direct · 2025
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Aiwei Ma *Department of Plastic Surgery and Burns Center, Second Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong, 515051, China.
Wenqi Shi *Department of Plastic Surgery and Burns Center, Second Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong, 515051, China.
Liyun Chen *Plastic Surgery Institute of Shantou University Medical College, Shantou, Guangdong, 515051, China.
Zijian Huang *Department of Plastic Surgery and Burns Center, Second Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong, 515051, China.
Yiwen ZhangDepartment of Plastic Surgery and Burns Center, Second Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong, 515051, China.
Zixuan TangDepartment of Plastic Surgery and Burns Center, Second Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong, 515051, China.
Wenshi JiangDepartment of Plastic Surgery and Burns Center, Second Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong, 515051, China.
Mengjing XuDepartment of Plastic Surgery and Burns Center, Second Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong, 515051, China.
Jianda ZhouDepartment of Plastic and Reconstructive Surgery, Central South University Third Xiangya Hospital, Changsha, China.
Wancong ZhangDepartment of Plastic Surgery and Burns Center, Second Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong, 515051, China. martine2007@sina.com.
Shijie TangDepartment of Plastic Surgery and Burns Center, Second Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong, 515051, China. sjtang3@stu.edu.cn.ORCID 0000-0002-5413-8828

Funding

2020 Li Ka Shing Foundation Cross-Disciplinary Research Grant 2020LKSFG02E2020 Li Ka Shing Foundation Cross-Disciplinary Research Grant 2020LKSFG18BGuangdong Basic and Applied Basic Research Foundation 2021A1515011142Guangdong Basic and Applied Basic Research Foundation 2023A1515012343, 2022A1515220099Guangdong University Innovation Team Project 2021KCXTD047National Natural Science Foundation of China 82071101Provincial science and technology innovation strategy special project funding program 210714106901245Provincial science and technology innovation strategy special project funding program STKJ202209067, STKJ2023004
6 · The paper itself

Abstract

backgroundIncreasing evidence suggests that long noncoding RNAs (lncRNAs) play important regulatory roles in biological processes and are dysregulated in numerous tumors. The lncRNA GRASLND functions as an oncogene in many cancers, but its role in skin cutaneous melanoma (SKCM) requires further investigation.

methodsSiRNA transfection, wound - healing and transwell assays were performed to evaluate the effect of GRASLND on cellular function.

resultsThe present study demonstrated that GRASLND expression is increased in SKCM tissues and cell lines. The high expression of GRASLND was correlated with poor prognosis and immunotherapy outcomes. Knockdown of GRASLND significantly inhibited cell migration and invasion. In addition, we found that miR-218-5p directly binds to its binding site on GRASLND, and GRASLND and miR-218-5p demonstrate mutual inhibition. Furthermore, the miR-218-5p inhibitor partially eliminated the knockdown of GRASLND and inhibited its expression. We also demonstrated that GRASLND acts as a miR-218-5p sponge that positively regulates STAM2 expression in SKCM cells.

conclusionIn summary, these data suggest that GRASLND functions by regulating miR-218-5p/STAM2 expression, suggesting an important role for the lncRNA‒miRNA-mRNA functional network and a new potential therapeutic target for SKCM.

Indexed as

Base SequenceCell MovementDisease ProgressionGene Expression Regulation, NeoplasticMelanomaMicroRNAsRNA, Long NoncodingAdaptor Proteins, Signal TransducingCell Line, TumorCell ProliferationEndosomal Sorting Complexes Required for TransportFemaleGene Knockdown TechniquesHumansMaleMiddle AgedAdaptor Proteins, Signal TransducingEndosomal Sorting Complexes Required for TransportMicroRNAsMIRN218 microRNA, humanRNA, Long NoncodingSTAM2 protein, humanBiomarkerImmunotherapylncRNAPrognosisSKCM

Identifiers

PMID39060946
PMCPMC11282654

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.