Evidence map›Paper›PMID 39060558›Full record

ArticleScientific reports2024

Tunneling nanotube-driven complete regeneration of murine fetal skin.

Yukari Nakajima, Shuichi Obata, Kento Takaya, Shigeki Sakai, Yushi Suzuki, Keisuke Okabe, Noriko Aramaki-Hattori, Ryoichi Mori, Yuichi Kadoya, Kazuo Kishi

Abstract read
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In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Yukari NakajimaDepartment of Plastic and Reconstructive Surgery, School of Medicine, Keio University, 35 Shinanomachi, Shinjukuku, Tokyo, Japan.
Shuichi ObataDepartment of Anatomical Sciences, School of Allied Health Sciences, Kitasato University, Kitasato 1-15-1, Minami-ku, Sagamihara, Kanagawa, 252-0373, Japan.
Kento TakayaDepartment of Plastic and Reconstructive Surgery, School of Medicine, Keio University, 35 Shinanomachi, Shinjukuku, Tokyo, Japan.
Shigeki SakaiDepartment of Plastic and Reconstructive Surgery, School of Medicine, Keio University, 35 Shinanomachi, Shinjukuku, Tokyo, Japan.
Yushi SuzukiDepartment of Plastic and Reconstructive Surgery, School of Medicine, Keio University, 35 Shinanomachi, Shinjukuku, Tokyo, Japan.
Keisuke OkabeDepartment of Plastic and Reconstructive Surgery, School of Medicine, Keio University, 35 Shinanomachi, Shinjukuku, Tokyo, Japan.
Noriko Aramaki-HattoriDepartment of Plastic and Reconstructive Surgery, School of Medicine, Keio University, 35 Shinanomachi, Shinjukuku, Tokyo, Japan.
Ryoichi MoriDepartment of Tissue Repair and Regenerative Medical Science, Atomic Bomb Disease Institute, Nagasaki University, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan.
Yuichi KadoyaDepartment of Anatomical Sciences, School of Allied Health Sciences, Kitasato University, Kitasato 1-15-1, Minami-ku, Sagamihara, Kanagawa, 252-0373, Japan.
Kazuo KishiDepartment of Plastic and Reconstructive Surgery, School of Medicine, Keio University, 35 Shinanomachi, Shinjukuku, Tokyo, Japan. kkishi@keio.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study investigated the three-dimensional (3D) cellular interactions and tunneling nanotubes (TNTs) during fetal mouse skin regeneration on embryonic days 13 (E13) and 15 (E15). We aimed to understand spatial relationships among cell types involved in skin regeneration and assess the potential role of TNTs. Full-thickness skin incisions were performed in E13 and E15 embryos. Wound sites were collected, embedded in epoxy resin, processed for 3D reconstruction (1 μm thickness sections), and subjected to whole-mount immunostaining. We conducted in vitro co-culture experiments with fetal macrophages and fibroblasts to observe TNT formation. To assess the effect of TNTs on skin regeneration, an inhibiting agent (cytochalasin B) was administered to amniotic fluid. Results revealed that E13 epidermal keratinocytes interacted with dermal fibroblasts and macrophages, facilitating skin regrowth. TNT structures were observed at the E13-cell wound sites, among macrophages, and between macrophages and fibroblasts, confirmed through in vitro co-culture experiments. In vitro and utero cytochalasin B administration hindered those formation and inefficient skin texture regeneration at E13 wound sites. This emphasizes the necessity of 3D cellular interactions between epidermal and dermal cells during skin regeneration in mouse embryos at E13. The prevalence of TNT structures indicated their involvement in achieving complete skin texture restoration.

Indexed as

Coculture TechniquesFibroblastsNanotubesRegenerationSkinAnimalsCell CommunicationCytochalasin BFemaleFetusKeratinocytesMacrophagesMiceWound HealingCytochalasin B

Identifiers

PMID39060558
PMCPMC11282213

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.