ArticleJournal of periodontal & implant science2024
Highly accurate measurement of the relative abundance of oral pathogenic bacteria using colony-forming unit-based qPCR.
Article in Journal of periodontal & implant science, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed.
- Arginine metabolism as a mutualistic strategy in the interaction between Porphyromonas gingivalis and Filifactor alocis.Nature communications · 2026Article
- Fermented cassava peel withVeterinary world · 2026Article
- Salivary Prevalence of Four Oral Pathogens in Postpartum Women in Northeast Romania: An Exploratory Cross-Sectional Study.Pathogens (Basel, Switzerland) · 2026Article
- Absolute quantification by mouthwash testing screens severe periodontitis and tracks species-specific microbial response to periodontal therapy.Frontiers in cellular and infection microbiology · 2026Article
- Application-focused validation of a viability-selective PMA-qPCR assay for selected skin-associated and cosmetic-relevant bacteria.Frontiers in microbiology · 2026Article
- Impact of Dexamethasone on Three-Dimensional Stem Cell Spheroids: Morphology, Viability, Osteogenic Differentiation.Medicina (Kaunas, Lithuania) · 2025Article
- Short-term effects of oral dissolving film on halitosis and oral pathogenic bacteria: a pilot non-randomized controlled trial.Frontiers in microbiology · 2025Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
purposeQuantitative polymerase chain reaction (qPCR) has recently been employed to measure the number of bacterial cells by quantifying their DNA fragments. However, this method can yield inaccurate bacterial cell counts because the number of DNA fragments varies among different bacterial species. To resolve this issue, we developed a novel optimized qPCR method to quantify bacterial colony-forming units (CFUs), thereby ensuring a highly accurate count of bacterial cells.
methodsTo establish a new qPCR method for quantifying 6 oral bacteria namely,
resultsFor each bacterium, the selected primer-probe set amplified serial-diluted standard templates indicative of bacterial CFUs. The resultant Ct values and the corresponding bacterial CFU values were used to construct a standard curve, the linearity of which was determined by a coefficient of determination (
conclusionsThe CFU-based qPCR quantification method provides highly accurate and reliable quantitation of oral pathogenic bacteria.
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What OpenQuestion holds
Registered trials
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