Evidence map›Paper›PMID 39058298›Full record

ArticleAngewandte Chemie (International ed. in English)2024

Standardized Parts for Activation of Small GTPase Signaling in Living Cells.

Yuchen He, Benjamin M Faulkner, Meaghan A Roberti, Dana K Bassford, Cliff I Stains

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Angewandte Chemie (International ed. in English), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Methods for Controlling Small GTPase Activity.Chembiochem : a European journal of chemical biology · 2025
    Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Yuchen HeDepartment of Chemistry, University of Virginia, Charlottsville, VA, 22904, USA.
Benjamin M FaulknerDepartment of Chemistry, University of Virginia, Charlottsville, VA, 22904, USA.
Meaghan A RobertiDepartment of Chemistry, University of Virginia, Charlottsville, VA, 22904, USA.
Dana K BassfordDepartment of Chemistry, University of Virginia, Charlottsville, VA, 22904, USA.
Cliff I StainsDepartment of Chemistry, University of Virginia, Charlottsville, VA, 22904, USA.ORCID 0000-0002-3165-4539

Funding

Chemical Approaches for Interrogating Fundamental Biomedical ProcessesR35GM148221 · NIGMS · UNIVERSITY OF VIRGINIA · PI Cliff I Stains · 2023 to 2026
$1.6M
Leica tauSTED super resolution microscopy for molecular imaging in fixed and live specimensS10OD030409 · OD · UNIVERSITY OF VIRGINIA · PI PERIASAMY, AMMASI · 2022 to 2022
$850k
NIGMS NIH HHS R35 GM148221NIGMS NIH HHS R35GM148221NIH HHS S10 OD030409NIH Office of the Director OD030409
6 · The paper itself

Abstract

Small GTPases comprise a superfamily of over 167 proteins in the human genome and are critical regulators of a variety of pathways including cell migration and proliferation. Despite the importance of these proteins in cell signaling, a standardized approach for controlling small GTPase activation within living cells is lacking. Herein, we report a split-protein-based approach to directly activate small GTPase signaling in living cells. Importantly, our fragmentation site can be applied across the small GTPase superfamily. We highlight the utility of these standardized parts by demonstrating the ability to directly modulate the activity of four different small GTPases with user-defined inputs, providing the first plug and play system for direct activation of small GTPases in living cells.

Indexed as

Monomeric GTP-Binding ProteinsSignal TransductionHEK293 CellsHumansMonomeric GTP-Binding ProteinsProtein engineeringSignal transductionSmall GTPasesSplit-protein reassembly

Identifiers

PMID39058298
PMCPMC12177683

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.