Evidence map›Paper›PMID 39057989›Full record

ArticleVeterinary sciences2024

Development and Clinical Application of a Molecular Assay for Four Common Porcine Enteroviruses.

Zhonghao Xin, Shiheng Li, Xiao Lu, Liping Liu, Yuehua Gao, Feng Hu, Kexiang Yu, Xiuli Ma, Yufeng Li, Bing Huang and 2 more

Abstract read
In one paragraph

Article in Veterinary sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Zhonghao XinKey Laboratory of Poultry Disease Diagnosis and Immunity in Shandong Province, Poultry Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China.ORCID 0009-0000-8183-6835
Shiheng LiKey Laboratory of Poultry Disease Diagnosis and Immunity in Shandong Province, Poultry Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Xiao LuKey Laboratory of Poultry Disease Diagnosis and Immunity in Shandong Province, Poultry Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Liping LiuKey Laboratory of Poultry Disease Diagnosis and Immunity in Shandong Province, Poultry Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Yuehua GaoKey Laboratory of Poultry Disease Diagnosis and Immunity in Shandong Province, Poultry Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Feng HuKey Laboratory of Poultry Disease Diagnosis and Immunity in Shandong Province, Poultry Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Kexiang YuKey Laboratory of Poultry Disease Diagnosis and Immunity in Shandong Province, Poultry Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Xiuli MaKey Laboratory of Poultry Disease Diagnosis and Immunity in Shandong Province, Poultry Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Yufeng LiKey Laboratory of Poultry Disease Diagnosis and Immunity in Shandong Province, Poultry Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Bing HuangKey Laboratory of Poultry Disease Diagnosis and Immunity in Shandong Province, Poultry Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Jiaqiang WuShandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan 250100, China.
Xiaozhen GuoKey Laboratory of Poultry Disease Diagnosis and Immunity in Shandong Province, Poultry Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Porcine epidemic diarrhea virus (PEDV), porcine transmissible gastroenteritis virus (TGEV), porcine deltacoronavirus (PDCoV), and porcine rotavirus-A (PoRVA) are the four main pathogens that cause viral diarrhea in pigs, and they often occur in mixed infections, which are difficult to distinguish only according to clinical symptoms. Here, we developed a multiplex TaqMan-probe-based real-time RT-PCR method for the simultaneous detection of PEDV, TGEV, PDCoV, and PoRVA for the first time. The specific primers and probes were designed for the M protein gene of PEDV, N protein gene of TGEV, N protein gene of PDCoV, and VP7 protein gene of PoRVA, and corresponding recombinant plasmids were constructed. The method showed extreme specificity, high sensitivity, and excellent repeatability; the limit of detection (LOD) can reach as low as 2.18 × 10

Indexed as

diagnosismultiplex real-time RT-PCRporcine enterovirusesTaqMan probe

Identifiers

PMID39057989
PMCPMC11281614

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.