Evidence map›Paper›PMID 39057809›Full record

ArticlePathogens (Basel, Switzerland)2024

Monoclonal Antibodies for Rift Valley Fever Virus Nucleocapsid: Application in IgG/IgM ELISA for Sero-Diagnosis.

Jiansheng Huang, Ferdinard Adungo, Samson Limbaso Konongoi, Shingo Inoue, Lin Zhan, Rosemary Sang, Salame Ashur, Allan Ole Kwallah, Matilu Mwau, Kouichi Morita and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Pathogens (Basel, Switzerland), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Jiansheng HuangSchool of Medicine, Guizhou University, Guiyang 550002, China.
Ferdinard AdungoKenya Medical Research Institute, Nairobi 54840-00200, Kenya.ORCID 0000-0002-0873-7993
Samson Limbaso KonongoiKenya Medical Research Institute, Nairobi 54840-00200, Kenya.
Shingo InoueDepartment of Virology, Institute of Tropical Medicine, Nagasaki University, 1-12-4, Sakamoto, Nagasaki 852-8523, Japan.
Lin ZhanNHC Key Laboratory of Pulmonary Immunological Diseases, Guizhou Provincial People's Hospital, Guiyang 550002, China.
Rosemary SangKenya Medical Research Institute, Nairobi 54840-00200, Kenya.
Salame AshurKenya Medical Research Institute, Nairobi 54840-00200, Kenya.
Allan Ole KwallahKenya Medical Research Institute, Nairobi 54840-00200, Kenya.ORCID 0000-0002-6459-3904
Matilu MwauKenya Medical Research Institute, Nairobi 54840-00200, Kenya.
Kouichi MoritaDepartment of Virology, Institute of Tropical Medicine, Nagasaki University, 1-12-4, Sakamoto, Nagasaki 852-8523, Japan.
Fuxun YuNHC Key Laboratory of Pulmonary Immunological Diseases, Guizhou Provincial People's Hospital, Guiyang 550002, China.ORCID 0000-0001-8804-2755

Funding

National Natural Science Foundation of China (2017) 81760605
6 · The paper itself

Abstract

introductionRift Valley fever virus (RVFV) belonging to the Phenuiviridae family is responsible for a zoonotic disease called Rift Valley fever (RVF). Currently, RVFV has spread from Africa to Asia, and due to its ability to cause high mortality rates, it has significantly impacted human health and economic development in many societies. Highly specific and sensitive systems for sero-diagnosis of RVFV infection are needed for clinical use.

methodBALB/c mice were immunized with recombinant RVFV nucleocapsid (rRVFV-N) protein and the spleen cells fused with SP2/0 myeloma cells to create hybridoma cell lines. The secreted monoclonal antibodies (MAbs) were purified and characterized. Enzyme-linked immunosorbent assay (ELISA) systems for the detection of IgG and IgM using the new MAbs were established and evaluated. Serum samples from 96 volunteers and 93 patients of suspected RVF from Kenya were tested compared with the ELISA systems based on inactivated viruses and the rabbit polyclonal antibody.

resultThree monoclonal antibodies against rRVFV-N protein were established. The performance of the MAb-based sandwich IgG ELISA and the IgM capture ELISA perfectly matched the ELISA systems using the inactivated virus or the polyclonal antibody.

conclusionsRecombinant RVFV-N protein-specific MAbs were developed and they offer useful tools for RVFV studies. The MAb-based ELISA systems for detecting IgG and IgM offer safe and useful options for diagnosing RVFV infections in humans.

Indexed as

Antibodies, MonoclonalAntibodies, ViralEnzyme-Linked Immunosorbent AssayImmunoglobulin GImmunoglobulin MMice, Inbred BALB CRift Valley FeverRift Valley fever virusAnimalsFemaleHumansKenyaMiceNucleocapsidNucleocapsid ProteinsRecombinant ProteinsAntibodies, MonoclonalAntibodies, ViralImmunoglobulin GImmunoglobulin MNucleocapsid ProteinsRecombinant ProteinsIgG ELISAIgM capture ELISAmonoclonal antibodynucleocapsid proteinRift Valley fever virus

Identifiers

PMID39057809
PMCPMC11279541

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.