Validation of Selected MicroRNA Transcriptome Data in the Bovine Corpus Luteum during Early Pregnancy by RT-qPCR.

Rreze M Gecaj, Behlul Behluli, Curtis R Youngs

Abstract read

In one paragraph

Article in Current issues in molecular biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Analysis of miRNA Expression in Bovine Endometrium During the Estrous Cycle.Animal science journal = Nihon chikusan Gakkaiho
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Rreze M GecajDepartment of Animal Biotechnology, Faculty of Agriculture and Veterinary, University of Pristina, 10000 Prishtina, Kosovo.ORCID 0000-0001-6909-8470

Behlul BehluliDepartment of Veterinary Medicine, Faculty of Agriculture and Veterinary, University of Prishtina, 10000 Pristina, Kosovo.

Curtis R YoungsDepartment of Animal Science, Iowa State University, Ames, IA 50011, USA.ORCID 0000-0003-4197-981X

Funding

This research was funded by the ME Ensminger Endowment at Iowa State University, the Uni-versity of Prishtina, and the European Commission (Tempus Project No. 1-2010-1-AT- TEMPUS-JPCR) 1-2010-1-AT- TEMPUS-JPCR

6 · The paper itself

Abstract

In cattle, the corpus luteum (CL) is pivotal in maintaining early pregnancy by secreting progesterone. To establish pregnancy, the conceptus produces interferon-τ, preventing luteolysis and initiating the transformation of the CL spurium into a CL verum. Although this transformation is tightly regulated, limited data are available on the expression of microRNAs (miRNAs) during and after this process. To address this gap, we re-analyzed previously published RNA-Seq data of CL from pregnant cows and regressed CL from non-pregnant cows. This analysis identified 44 differentially expressed miRNAs. From this pool, three miRNAs-bta-miR-222-3p, bta-miR-29c, and bta-miR-2411-3p-were randomly selected for relative quantification. Using bovine ovaries (n = 14) obtained from an abattoir, total RNA (including miRNAs) was extracted and converted to cDNA for RT-qPCR. The results revealed that bta-miR-222-3p was downregulated (

Indexed as

cattlecorpus albicanscorpus luteummicroRNANGSpregnancyRT-qPCR

Identifiers

PMID39057036

DOI10.3390/cimb46070394

PMCPMC11275921

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LicenceCC BY

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.