Evidence map›Paper›PMID 39055761›Full record

ArticleOpen veterinary journal2024

Designing a multiepitope mRNA-based vaccine against enterotoxigenic

Zainab Isam Alawadi, Amjed Alsultan, Dhama Alsallami, Sultan F Alnomasy, Mohammed Alqasmi, Fawaz M Almufarriji, Bader S Alotaibi, Bi Bi Zainab Mazhari, Rawaf Alenazy

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Article in Open veterinary journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Zainab Isam AlawadiDepartment of Internal and Preventive Medicine, College of Veterinary Medicine, University of Al-Qadisiyah, Al- Diwaniyah, Iraq.
Amjed AlsultanDepartment of Internal and Preventive Medicine, College of Veterinary Medicine, University of Al-Qadisiyah, Al- Diwaniyah, Iraq.
Dhama AlsallamiDepartment of Physiology, Pharmacology and Biochemistry, College of Veterinary Medicine, University of Al-Qadisiyah, Al-Diwaniyah, Iraq.
Sultan F AlnomasyMedical Laboratories Department, College of Applied Medical Sciences in Al- Quwayiyah, Shaqra University, Shaqra, Saudi Arabia.
Mohammed AlqasmiDepartment of Medical Laboratory, College of Applied Medical Sciences-Shaqra, Shaqra University, Shaqra, Saudi Arabia.
Fawaz M AlmufarrijiMedical Laboratories Department, College of Applied Medical Sciences in Al- Quwayiyah, Shaqra University, Shaqra, Saudi Arabia.
Bader S AlotaibiMedical Laboratories Department, College of Applied Medical Sciences in Al- Quwayiyah, Shaqra University, Shaqra, Saudi Arabia.
Bi Bi Zainab MazhariDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakakah, Saudi Arabia.
Rawaf AlenazyDepartment of Medical Laboratory, College of Applied Medical Sciences-Shaqra, Shaqra University, Shaqra, Saudi Arabia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Aim: Current work is aimed at designing an effective and safe multiepitope vaccine candidate against Methods: A conserved sequence of K99 protein was generated, and then highly antigenic, nonallergic, and overlapped epitopes were used to construct a multiepitope vaccine. Five THL, six MHC II, and four beta cell epitopes were targeted to create the candidate. The candidate vaccine was produced utilizing 15 epitopes and three types of linkers, two types of untranslated region (UTR) human hemoglobin subunit beta (HBB), UTR beta-globin (Rabb), and RpfE protein as an immunomodulation adjuvant. Results: Immuno-informatics analysis of the constructed protein showed that the protein was antigenic (antigenic score of 0.8841), stable, nonallergen, and soluble. Furthermore, the Immuno-informatics and physiochemical analysis of the constructed protein showed a stable, nonallergic, soluble, hydrophilic, and acidic PI (isoelectric point). of 9.34. Docking of the candidate vaccine with the toll-like receptor TLR3 was performed, and results showed a strong interaction between the immune receptor and the vaccine. Finally, the expression efficiency of the construct in Conclusion: Results of immunoinformatics and

Indexed as

Cattle DiseasesComputational BiologyEnterotoxigenic Escherichia coliEscherichia coli InfectionsEscherichia coli VaccinesAnimalsCattleEpitopesImmunoinformaticsModels, MolecularRNA, MessengerEpitopesEscherichia coli VaccinesRNA, MessengerEnterotoxigenic E. coliFimbrial protein K99mRNA vaccineMultiepitope vaccineNeonatal calf diarrhea

Identifiers

PMID39055761
PMCPMC11268904

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.