ArticleThe journal of venomous animals and toxins including tropical diseases2024
An improved high-performance liquid chromatography (HPLC) method for detection of variations in the hydroxyproline content of tissue homogenates from
Article in The journal of venomous animals and toxins including tropical diseases, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Background: Paracoccidioidomycosis (PCM) is a severe granulomatous disease Methods: Five B10.A female mice were infected with Results: Infected mice showed numerous, disseminated paracoccidioidomycotic lesions, as well as marked collagen deposits, as observed in histopathologic analysis, and high levels of HYP. Normal uninfected mice showed no granulomas, little or no deposits of collagen fibers, and very low levels of HYP, as evaluated by HPLC. Our results show that the disease intensity as evaluated number and the morphology of the granulomatous lesions were correlated to the HYP levels using small tissue samples from the omentum, the main target organ of Conclusions: Here we propose an alternative methodology to follow disease evolution and, to some extent, fungal load in experimental
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